We narrowed to 10,031 results for: pET

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pET29b_SpyTag003-L-VHH21-His

Plasmid

#233333

Purpose

To express nanobody VHH21 with N terminal SpyTag

Depositor

Magnus Rueping

Publication

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-VHH21-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET24+ CUL5(1-384)

Plasmid

#246504

Purpose

Recombinant protein expression of CUL5(1-384)

Depositor

Steven Clarke

Publication

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

cullin-5 (CUL5 Human)

Expression

Bacterial

Mutation

Deleted amino acids 385-780

Available since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET15b-TGP-TwinStrep

Plasmid

#240251

Purpose

IPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tags

Depositor

Olga Boudker, Krishna Reddy

Publication

Reddy et al Nat Struct Mol Biol (2025).

Type

Empty backbone

Tags

10x HIS, Thrombin, Twin-Strep, and thermostable G…

Expression

Bacterial

Promoter

T7

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET15b-ALFA-TwinStrep

Plasmid

#240252

Purpose

IPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tags

Depositor

Olga Boudker, Krishna Reddy

Publication

Reddy et al Nat Struct Mol Biol (2025).

Type

Empty backbone

Tags

10x HIS, ALFA, Thrombin, and Twin-Strep

Expression

Bacterial

Promoter

T7

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-Hf-RfxCas13d-His

Plasmid

#234480

Purpose

Plasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Publication

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

RfxCas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Changed four alanine to valine in positions 134, …

Available since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b+R3(R112C/T180C)

Plasmid

#108195

Purpose

Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Publication

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA3(R112C/T180C) fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET11d-FHis-DND1

Plasmid

#70083

Purpose

Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein. Codons are optimized for bacteria expression.

Depositor

Thomas Tuschl

Publication

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (DND1 Human)

Tags

6xHIS and FLAG

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 L171C

Plasmid

#66177

Purpose

Expresses the L171C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Publication

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Mutation

L171C

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 P186C

Plasmid

#66173

Purpose

Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Publication

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Mutation

P186C

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 G108C

Plasmid

#66140

Purpose

Expresses the G108C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Publication

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Mutation

G108C

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET11a+D77R/T214V

Plasmid

#66135

Purpose

Expresses the D77R/T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Publication

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET28(a)-hFX(I154F)-HA

Plasmid

#14992

Depositor

Michael Ristow

Insert

human frataxin cDNA (FXN Human)

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pET28a-AncMEcoGII291

Plasmid

#205024

Purpose

Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

Depositor

Paola Laurino

Publication

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

Ancestral M.EcoGII 291

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher

Plasmid

#223633

Purpose

Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His6

Expression

Bacterial

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-DuraPETase

Plasmid

#218699

Purpose

Encodes codon optimized DuraPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

DuraPETase

Tags

10xHis

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET47b-T7-enAsCas12f

Plasmid

#204642

Purpose

Expression of enAsCas12f in E. coli

Depositor

Weixin Tang

Publication

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

enAsCas12f

Tags

6xHis

Expression

Bacterial

Mutation

D196K, N199K, G276R, N328G, D364R

Promoter

T7

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET24b-Amg2

Plasmid

#199434

Purpose

expresses Amg2 in bacterial cells

Depositor

Andrew Woolley

Publication

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

Amg2

Tags

his tag

Expression

Bacterial

Mutation

L159K mutation

Promoter

T7

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-41-HsPABPC1_J

Plasmid

#146644

Purpose

Bacterial Expression of HsPABPc1

Depositor

Elisa Izaurralde

Publication

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsPABPc1 (PABPC1 Human)

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET41sIII_GST-ALC1

Plasmid

#173932

Purpose

Bacterial expression of ALC1

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1 (CHD1L Human)

Tags

GST

Expression

Bacterial

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-EGFP

Plasmid

#165040

Purpose

Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

Depositor

Dokyun Na

Publication

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

EGFP

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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