We narrowed to 7,305 results for: plasmid dna

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pspCas9-POLI-ST2-com vector

Plasmid

#176234

Purpose

Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Type

Empty backbone

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pJSM101

Plasmid

#48791

Purpose

NHEJ reporter plasmid for 5' and 3' cohesive ends

Depositor

Michael Glickman

Article

Zhu et al Biochemistry. 2012 Dec 21;51(51):10147-5

Insert

split lacZ

Expression

Bacterial

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pJSM103

Plasmid

#48872

Purpose

NHEJ reporter plasmid for blunt ends

Depositor

Michael Glickman

Article

Zhu et al Biochemistry. 2012 Dec 21;51(51):10147-5

Insert

split lacZ

Expression

Bacterial

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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SAGA005

Plasmid

#240754

Purpose

Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA p15A J23100 BCD_medium - gfp - tetAp15A- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGBW-m4092709

Plasmid

#153859

Purpose

Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Bacterial

Mutation

Nearly native encoding;handful SNPs as watermarks…

Promoter

Promoter | t7 consensus from NEB, G is +1

Available Since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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SAGA046

Plasmid

#240795

Purpose

Plasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA pBR322ROP His medium : T7- His tags medium- gfp -pBR322ROP tetApBR322ROP - CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA045

Plasmid

#240794

Purpose

Plasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA pBR322ROP His low : T7- His tags low - gfp -pBR322ROP tetApBR322ROP - CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA043

Plasmid

#240792

Purpose

Plasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA p15a His medium : T7- His tags medium- gfp -p15a tetAp15a - CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA042

Plasmid

#240791

Purpose

Plasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA p15a His low : T7- His tags low - gfp -p15a tetAp15a - CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA040

Plasmid

#240789

Purpose

Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 His medium : T7- His tags medium- gfp -RK2 tetARK2 - CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA039

Plasmid

#240788

Purpose

Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 His low : T7- His tags low - gfp -RK2 tetARK2 - CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA032

Plasmid

#240781

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA031

Plasmid

#240780

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA030

Plasmid

#240779

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_low - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA023

Plasmid

#240772

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA022

Plasmid

#240771

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA021

Plasmid

#240770

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_low - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SAGA019

Plasmid

#240768

Purpose

Plasmid contains the triple selection cassette gfp -tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA pBR322-ROP PrhaBAD BCD_medium - gfp -tetApBR322-ROP- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA018

Plasmid

#240767

Purpose

Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA pBR322-ROP PrhaBAD BCD_low - gfp - tetApBR322-ROP- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA015

Plasmid

#240764

Purpose

Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA p15A PrhaBAD BCD_low - gfp - tetAp15A- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge