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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,882 results for: ENA

Showing: 1641 - 1660 of 12882 results

  3. pRL488

    Plasmid
    #70263
    Purpose
    designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.
    Depositor
    Peter Wolk
    Article
    Elhai et al EMBO J. 1990 Oct;9(10):3379-88.
    Type
    Empty backbone
    Use
    Luciferase; Cyanobacteria expression
    Tags
    luxAB
    Expression
    Mutation
    Promoter
    none
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70263-plasmid-map-sequence-id-130206

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  4. pJK372

    Plasmid
    #73597
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73597-plasmid-map-sequence-id-138754

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  5. pJK370

    Plasmid
    #73595
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 2)
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Silent mutation in Arg299 codon.
    Promoter
    GAL1 and GAL10
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73595-plasmid-map-sequence-id-138462

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  7. pET28a MDHM_YEAST

    Plasmid
    #204231
    Purpose
    Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHM_YEAST (MDH1 Budding Yeast)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204231-plasmid-map-sequence-id-413335

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  10. pET28a-Ec.coaA

    Plasmid
    #50386
    Purpose
    Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.
    Depositor
    Erick Strauss
    Article
    Brand et al J Biol Chem. 2005 May 27;280(21):20185-8
    Insert
    PanK
    Use
    Tags
    6XHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    50386-plasmid-map-sequence-id-75331

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  14. pQE60 MGH

    Plasmid
    #204244
    Purpose
    Bacterial expression of a fusion of watermelon glyoxysomal malate dehydrogenase (MDH) and GFP with His tag; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MGH
    Use
    Tags
    6X His and GFP
    Expression
    Bacterial
    Mutation
    fusion of MDH and GFP with His tag
    Promoter
    Lac
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204244-plasmid-map-sequence-id-411051

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Showing: 1641 - 1660 of 12882 results