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pX330-U6-W308_guide-CBh-hSpCas9

Plasmid

#188546

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Promoter

U6

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-U6-L101_guide-CBh-hSpCas9

Plasmid

#188547

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Promoter

U6

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) R-Ras1(G38C)

Plasmid

#224286

Purpose

Cell transfection and expression of R-Ras1 (G38C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

R-Ras1 (RRAS Human)

Expression

Mammalian

Mutation

G38C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI2B-3×FLAG

Plasmid

#215510

Purpose

Expresses FLAG tagged human WIPI2B.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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H1DBD-Halo-HA

Plasmid

#200203

Purpose

Expression of truncated HSF1 (DBD and HR)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

H1DBD-Halo-HA (Hsf1 Mouse)

Tags

Halo; HA

Expression

Mammalian

Mutation

HSF1 (1-399)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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H1DBD-HR-Halo

Plasmid

#200204

Purpose

Expression of truncated HSF1 (DBD and HR)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

H1DBD-HR-Halo (Hsf1 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

HSF1 (1-672)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgFADD guide 1

Plasmid

#193589

Purpose

FADD knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgFADD guide 1 (FADD Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgFADD guide 2

Plasmid

#193590

Purpose

FADD knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgFADD guide 2 (FADD Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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px459-H3.3 (H3f3b) sgRNA

Plasmid

#186931

Purpose

H3f3b sgRNA used for genomic targeting at H3f3b C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3b sgRNA (H3f3b Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn1.1_Kctd19-3xHA

Plasmid

#173497

Purpose

Express Kctd19-3xHA under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Article

Oura et al PLoS Genet. 2021 May 7;17(5):e1009412. d

Insert

Potassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pClgn1.1_3xFLAG-Kctd19

Plasmid

#173498

Purpose

Express 3xFLAG-Kctd19 under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Article

Oura et al PLoS Genet. 2021 May 7;17(5):e1009412. d

Insert

Potassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-dt/deltaEGFP

Plasmid

#163919

Purpose

PGK-driven dTomato expression. Lacks the CBA-promoter controlling EGFP expression.

Depositor

John Engelhardt

Article

Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

Insert

dtomato

Use

Lentiviral

Expression

Mammalian

Mutation

WT

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_RtoK

Plasmid

#139120

Purpose

express hnRNPA2 LC where all R are K

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all R to K

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_NtoS

Plasmid

#139121

Purpose

express hnRNPA2 with all N changed to S

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all N to S

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_5E

Plasmid

#139122

Purpose

express hnRNPA2 with 5 serine changed to glutamate

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

5 serine to glutamate

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_12E

Plasmid

#139123

Purpose

express hnRNPA2 with all serine change to glutamate

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

12 S to E

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPF_PLD_YtoS

Plasmid

#139124

Purpose

express hnRNPF PLD with all Y changed to S

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all Y to S

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPF_PLD_StoA

Plasmid

#139125

Purpose

express hnRNPF PLD with all S changed to A

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all S to A

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA INPP5E KD2

Plasmid

#162005

Purpose

INPP5E shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP5E (INPP5E Canis Lupus)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOK6_IRS

Plasmid

#109886

Purpose

Protein expression and purification of DOK6_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DOK6_IRS (DOK6 Human)

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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