We narrowed to 2,005 results for: CCS
-
Plasmid#208343PurposepLentiCRISPRv2-Blast backbone with two separate sgRNAs against AAVS1. Serves as a negative control by targeting a safe harbor locus.DepositorArticleAvailable SinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only
-
pCR8-CDK12(sgR)
Plasmid#208346PurposeGateway cloning entry vector with CDK12 with sgRNA resistant silent mutations. Must be recombined into a DEST vector for expression.DepositorArticleInsertcyclin dependent kinase 12 (CDK12 Human)
UseGateway: entry vectorMutationSilent mutations in A88, K90, D92, R94, T715, S71…Available SinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgStuffer-GFP-Puro
Plasmid#208349PurposeLentivirus sgRNA backbone with GFP and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)DepositorArticleTypeEmpty backboneUseCRISPR and LentiviralPromoterhU6Available SinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgStuffer-mCherry-Puro
Plasmid#208350PurposeLentivirus sgRNA backbone with mCherry and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)DepositorArticleTypeEmpty backboneUseCRISPR and LentiviralPromoterhU6Available SinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
FUCGW-DEST
Plasmid#208408PurposeLentivirus compatible with LR Gateway cloning for UBC-driven gene expression with CMV-GFP in backboneDepositorArticleTypeEmpty backboneUseLentiviral; Gateway: dest vectorPromoterUBCAvailable SinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
FUCRW-DEST
Plasmid#208409PurposeLentivirus compatible with LR Gateway cloning for UBC-driven gene expression with CMV-RFP in backboneDepositorArticleTypeEmpty backboneUseLentiviral; Gateway: dest vectorPromoterUBCAvailable SinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV TRE-Tight CheRiff-mKate2
Plasmid#182493PurposeExpression of CheRiff channelrhodopsin linked to mKate2 from a Tet-inducible promoterDepositorInsertCheRiff (#51697 Adam Cohen Lab)
UseAAVTagsmKate2 (Evrogen)ExpressionMammalianPromoterTRE-Tight (Clontech)Available SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-SAM-Puro
Plasmid#198713PurposeFor expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.DepositorInsertsU6-MS2-gRNAscaffold
EF1a-MPH-Puro
UseCRISPR and LentiviralExpressionMammalianPromoterEF1a and U6Available SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_G1A-T2A_R
Plasmid#200183PurposeWT PA14 I-F Leader and G1A-T2A RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (G1A-T2A)
ExpressionBacterialMutationG1A-T2A in the I-F repeatAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_A28C_R
Plasmid#200184PurposeWT PA14 I-F Leader and A28C RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (A28C)
ExpressionBacterialMutationA28C in the I-F repeatAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_G1A-T2A-A28C_R
Plasmid#200185PurposeWT PA14 I-F Leader and G1A-T2A-A28C RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
ExpressionBacterialMutationG1A-T2A-A28C in the I-F repeatAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-457
Plasmid#200213PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)DepositorInsertPaCas1, PaCas2-3
TagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 H25A mutationAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-458
Plasmid#200214PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)DepositorInsertPaCas1, PaCas2-3
TagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 E184A mutationAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-459
Plasmid#200215PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)DepositorInsertPaCas1, PaCas2-3
TagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 K214A mutationAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-460
Plasmid#200216PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)DepositorInsertPaCas1, PaCas2-3
TagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 R55E-N56D mutationsAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-461
Plasmid#200217PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)DepositorInsertPaCas1, PaCas2-3
TagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E mutationsAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-462
Plasmid#200218PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)DepositorInsertPaCas1, PaCas2-3
TagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E, R55E-N56D mutationsAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)
Plasmid#208974PurposeRecruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertB103-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianPromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)
Plasmid#208973PurposeRecruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertePhi29-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)
Plasmid#208972PurposeRecruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertePhi29(+exo)-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only