We narrowed to 15,982 results for: LEA

1. pZW1-snoVector

   Plasmid

   #73174

   Purpose

   Nuclear expression of RNAs

   Depositor

   Ling-Ling Chen

   Article

   Yin et al Nucleic Acids Res. 2015 Jan;43(1):e5. do

   Inserts

   SNORD116-13 (SNORD116-13 Human)
   SNORD116-14 (SNORD116-14 Human)
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJS96

   Plasmid

   #207588

   Purpose

   GFP-TonBH20A expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-TonBH20A
   

   Expression

   Bacterial

   Mutation

   H20A

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJS119

   Plasmid

   #207592

   Purpose

   GFP-TonB deltaII expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-TonB deltaII
   

   Expression

   Bacterial

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJS104

   Plasmid

   #207589

   Purpose

   GFP-TolA deltaII expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-TolA deltaII
   

   Expression

   Bacterial

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJS70

   Plasmid

   #207586

   Purpose

   GFP-TolA H22A expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-TolA H22A
   

   Expression

   Bacterial

   Mutation

   H22A

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSP618

   Plasmid

   #169235

   Purpose

   Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

   Depositor

   William Greenleaf

   Article

   Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

   Insert

   mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. hMSH2 Clone E3 (full-length)

   Plasmid

   #16453

   Depositor

   Bert Vogelstein

   Article

   Leach et al Cell. 1993 Dec 17. 75(6):1215-25.

   Insert

   MSH2 (MSH2 Human)
   

   Expression

   Bacterial

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. Venus-tagged Apollo-NADP+

   Plasmid

   #71797

   Purpose

   Expresses Venus-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

   Depositor

   Jonathan Rocheleau

   Article

   Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

   Insert

   Apollo-NADP+
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-NFIA1.1

   Plasmid

   #112698

   Purpose

   Expression of NFIA1.1 protein for transient transfection assays

   Depositor

   Richard Gronostajski

   Article

   Chaudhry et al J Biol Chem. 1999 Mar 12;274(11):7072-81

   Insert

   Nuclear Factor I A1.1 (Nfia Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PRDX6A-c001

    Plasmid

    #175474

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    PRDX6 (PRDX6 Human)
    

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    See Depositor Comments

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid

    #40132

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease (ELD)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid

    #40131

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease (KKR)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV0679

    Plasmid

    #149689

    Purpose

    Bacterial expression of SENP_EuH protease

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SENP_EuH protease
    

    Tags

    His14-tag and Tev protease cleavage site

    Expression

    Bacterial

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57-mcGAS-LL/RK-NLS/D307A

    Plasmid

    #185459

    Purpose

    Inducible nuclear cGAS with D307A mutation

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    L157R, L159K, D307A

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. H2B-mScarlet- hMeikin(1-332)-mNeonGreen

    Plasmid

    #174714

    Purpose

    Stable expression of fluorescent Separase cleavage sensor with human Meikin

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin
    

    Use

    Retroviral

    Tags

    H2B-mScarlet and mNeonGreen

    Expression

    Mammalian

    Mutation

    1-332

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

    Plasmid

    #128749

    Purpose

    Expresses control gRNA

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. CD44Z-c011

    Plasmid

    #175455

    Purpose

    Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Tags

    His6

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMBP-LbCas12a D832A

    Plasmid

    #113517

    Purpose

    Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    LbCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    D832A

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-T3-eSpCAS9-NG-pA

    Plasmid

    #131466

    Purpose

    Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

    Insert

    Codon optimized eSpCas9-NG
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. CD44I-c013

    Plasmid

    #175450

    Purpose

    Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Tags

    His6

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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