We narrowed to 15,785 results for: LEA

1. pAV0286

   Plasmid

   #149333

   Purpose

   Bacterial expression of SENP_EuB protease

   Depositor

   Dirk Görlich

   Article

   Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

   Insert

   SENP_EuB protease
   

   Use

   Tags

   His14 and Tev protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJS96

   Plasmid

   #207588

   Purpose

   GFP-TonBH20A expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-TonBH20A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H20A

   Promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJS119

   Plasmid

   #207592

   Purpose

   GFP-TonB deltaII expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-TonB deltaII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJS104

   Plasmid

   #207589

   Purpose

   GFP-TolA deltaII expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-TolA deltaII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJS70

   Plasmid

   #207586

   Purpose

   GFP-TolA H22A expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-TolA H22A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H22A

   Promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZW1-snoVector

   Plasmid

   #73174

   Purpose

   Nuclear expression of RNAs

   Depositor

   Ling-Ling Chen

   Article

   Yin et al Nucleic Acids Res. 2015 Jan;43(1):e5. do

   Inserts

   SNORD116-13 (SNORD116-13 Human)
   SNORD116-14 (SNORD116-14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmCherry-C1 mCherry-NLS

   Plasmid

   #58476

   Purpose

   Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Insert

   3X NLS with 3X stop codons
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPSup_iGFP

   Plasmid

   #149420

   Purpose

   Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter. The GFP reporter gene harbors the IV2 intron.

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET22b RNase A

   Plasmid

   #58903

   Purpose

   Bacterial expression of bovine pancreatic ribonuclease A

   Depositor

   Ronald Raines

   Article

   delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

   Insert

   RNase A (RNASE1 Bovine)
   

   Use

   Tags

   pelB sequence

   Expression

   Bacterial

   Mutation

   See Depositor Comments below

   Promoter

   T7

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSP618

    Plasmid

    #169235

    Purpose

    Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

    Depositor

    William Greenleaf

    Article

    Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmCherry-C1-RanQ69L

    Plasmid

    #30309

    Depositor

    Jay Brenman

    Article

    Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

    Insert

    RanQ69L (RAN N/A)
    

    Use

    Tags

    FLAG-Cherry

    Expression

    Mammalian

    Mutation

    Glutamine 69 is changed to Leucine

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hMSH2 Clone E3 (full-length)

    Plasmid

    #16453

    Depositor

    Bert Vogelstein

    Article

    Leach et al Cell. 1993 Dec 17. 75(6):1215-25.

    Insert

    MSH2 (MSH2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Venus-tagged Apollo-NADP+

    Plasmid

    #71797

    Purpose

    Expresses Venus-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

    Depositor

    Jonathan Rocheleau

    Article

    Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

    Insert

    Apollo-NADP+
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-NFIA1.1

    Plasmid

    #112698

    Purpose

    Expression of NFIA1.1 protein for transient transfection assays

    Depositor

    Richard Gronostajski

    Article

    Chaudhry et al J Biol Chem. 1999 Mar 12;274(11):7072-81

    Insert

    Nuclear Factor I A1.1 (Nfia Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PRDX6A-c001

    Plasmid

    #175474

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    PRDX6 (PRDX6 Human)
    

    Use

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    See Depositor Comments

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCW57-mcGAS-LL/RK-NLS/D307A

    Plasmid

    #185459

    Purpose

    Inducible nuclear cGAS with D307A mutation

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    L157R, L159K, D307A

    Promoter

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid

    #40132

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Use

    Tags

    FokI nuclease (ELD)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid

    #40131

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Use

    Tags

    FokI nuclease (KKR)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV0679

    Plasmid

    #149689

    Purpose

    Bacterial expression of SENP_EuH protease

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SENP_EuH protease
    

    Use

    Tags

    His14-tag and Tev protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. H2B-mScarlet- hMeikin(1-332)-mNeonGreen

    Plasmid

    #174714

    Purpose

    Stable expression of fluorescent Separase cleavage sensor with human Meikin

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin
    

    Use

    Retroviral

    Tags

    H2B-mScarlet and mNeonGreen

    Expression

    Mammalian

    Mutation

    1-332

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge