Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 4,418 results for: Pet2

Showing: 1661 - 1680 of 4418 results

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pET28b-NmCas9-Flag H588A

Plasmid

#109032

Purpose

H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-EC12-129/138

Plasmid

#47926

Purpose

Expresses E-cadherin EC12 with C9A, K129C/D138C mutations in E.Coli.

Depositor

Tanja Kortemme

Article

Ritterson et al J Am Chem Soc. 2013 Aug 7.

Insert

E-Cadherin EC12

Tags

6xHis and TEV Cleavage

Expression

Bacterial

Mutation

Mutations C9A, K129C, D138C

Promoter

T7

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_ModularAssembly&activeSite

Plasmid

#248013

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&ActiveSite design)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly&activeSite

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_WT-LGP2 (codon optimized)

Plasmid

#248163

Purpose

Expresses full-length human LGP2 in E. coli

Depositor

Smita Patel

Article

Lee et al Nucleic Acids Res. 2024 Jan 11;52(1):355

Insert

DExH-box helicase 58 (DHX58) (DHX58 Human)

Tags

6xHis, SUMO

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28 His-SUMO-CHMP2B

Plasmid

#232018

Purpose

Bacterial expression of His and SUMO tagged CHMP2B

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

6x His, SUMO

Expression

Bacterial

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-His6-UROD-N367A

Plasmid

#236740

Purpose

Protein overexpression of human uroporphyrinogen decarboxylase N367A

Depositor

Christina Woo

Article

Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

Insert

Uroporphyrinogen decarboxylase human N367A (UROD Human)

Tags

His6

Expression

Bacterial

Mutation

N367A

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-His6-GFP-UROD

Plasmid

#236741

Purpose

Protein overexpression of eGFP-human uroporphyrinogen decarboxylase

Depositor

Christina Woo

Article

Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

Insert

eGFP-Uroporphyrinogen decarboxylase human (UROD Human, Synthetic)

Tags

His6 and eGFP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) E83A

Plasmid

#240583

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, E83A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K80A

Plasmid

#240584

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K80A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K96A

Plasmid

#240585

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K96A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K97A

Plasmid

#240586

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K97A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K102A

Plasmid

#240588

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K102A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) D98A

Plasmid

#240587

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, D98A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET23a-His-TEV-SpyCatcher3

Plasmid

#235149

Purpose

Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

SpyCatcher3

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET29_anti-SARS-CoV-2-NM1226

Plasmid

#209422

Purpose

Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1226

Tags

Myc-6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-NM1230

Plasmid

#209423

Purpose

Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1230

Tags

Myc-6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-MERS-Nanobody-VHH-55

Plasmid

#209417

Purpose

Express anti-MERS-Nanobody-VHH-55 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-mers-nanobody-vhh-55

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-MERS-Nanobody-VHH-83

Plasmid

#209418

Purpose

express anti-MERS-Nanobody-VHH-83 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-mers-nanobody-vhh-83

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-MERS-Nanobody-VHH-84

Plasmid

#209419

Purpose

Express anti-MERS-Nanobody-VHH-84 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-mers-nanobody-vhh-83

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-Nanobody-C5

Plasmid

#209420

Purpose

Express anti-SARS-CoV-2-Nanobody C5 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-Nanobody-C5

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

Showing: 1661 - 1680 of 4418 results

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