Purpose
              
              hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
             
          
          
          
            
          
        
          
  
  
    
      
        Inserts
      
      
        
          HH - FS23 linker - HDV
        
          human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
        
      
    
   
  
  
    
  
  
  
    
  
  
  
         
          
            
              
                Available Since
              
              
                April 8, 2019
              
             
          
          
            
              Availability
            
            
              
                Academic Institutions and Nonprofits only