We narrowed to 2,564 results for: LRE
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Plasmid#176071PurposeEGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassetteDepositorInsertRG
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo R99A nsp1
Plasmid#175422PurposeExpresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 R99A nsp1
Tags3XFLAG HaloExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-MCS-LexA-VP16-MiniWhite
Plasmid#165900PurposeBlasticidin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42 -SSRP1
Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO ACD11
Plasmid#170738PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA2 R2896C
Plasmid#139511PurposePlasmid expressing a sgRNA to introduce BRCA2 R2896C using base editingDepositorInsertsgRNA to insert BRCA2 E2896C using base editing
ExpressionMammalianPromoterU6Available SinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-Y530EEI
Plasmid#140322PurposeExpresses HA-tagged human Src-Y530EEI in mammalian cells for structure-function studiesDepositorAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-UD
Plasmid#140305PurposeExpresses Flag-tagged human Src-S4-UD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X FlagExpressionMammalianMutationcontains only the SH4 and UD regionsPromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-UD-KD
Plasmid#140301PurposeExpresses Flag-tagged human Src-S4-UD-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X FlagExpressionMammalianMutationlacks the SH3 and SH2 domainsPromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Rictor-g1)-PGKpuroBFP-W
Plasmid#105041PurposeLentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Rictor-g2)-PGKpuroBFP-W
Plasmid#105042PurposeLentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tcf7l1-g1)-PGKpuroBFP-W
Plasmid#105015PurposeLentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFPDepositorInsertgRNA targeting Tcf7l1 (Tcf7l1 Mouse)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Pggt1b-g1)-PGKpuroBFP-W
Plasmid#105018PurposeLentiviral gRNA plasmid targeting mouse Pggt1b , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Znrf1-g1)-PGKpuroBFP-W
Plasmid#105020PurposeLentiviral gRNA plasmid targeting mouse Znrf1 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11D)-mPAGFP
Plasmid#114135PurposeMammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
ExpressionMammalianAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_m-AKAR5
Plasmid#114139PurposeMammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 C-terminally tagged by KRas C tail
ExpressionMammalianAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cyt-AKAR5
Plasmid#114138PurposeMammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 with C-terminal nuclear export signal (NES)
ExpressionMammalianAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11A)-mPAGFP
Plasmid#114134PurposeMammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
ExpressionMammalianAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN-mPAGFP
Plasmid#114114PurposeMammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
ExpressionMammalianAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
vsv-Incenp 1-48-GFP
Plasmid#108507Purposeexpression of vsv-INCENP 1-48-EGFPDepositorInsertCEN-Box of INCENP (INCENP Human)
TagsEGFP and VSVExpressionMammalianMutationonly CEN box of INCENPPromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only