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Plasmid#153907PurposeBacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stopDepositorInsertfragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationwtPromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-lynD3cpV
Plasmid#37472DepositorInsertD3cpv second generation cameleon (calcium sensor)
UseTagsthe myristoylation and palmitoyla- tion sequence …ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YWHAQ_14-3-3
Plasmid#109943PurposeProtein expression and purification of YWHAQ_14-3-3DepositorInsertYWHAQ_14-3-3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPTD-DRBD
Plasmid#35622DepositorInsertPKR DRBD-1
UseTags6xHIS, HA, and TATExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YWHAE_14-3-3
Plasmid#109942PurposeProtein expression and purification of YWHAE_14-3-3DepositorInsertYWHAE_14-3-3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_pMOX
Plasmid#160922PurposeMethanol oxidase (MOX) promoter module for episomal P. pastoris promoter shufflingDepositorInsertMethanol oxidase promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Aurein1.2_(pos36GFP)-Cys
Plasmid#71757PurposeAurein1.2_+36GFP-Cys expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-Cys
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKB239
Plasmid#170034PurposeEncodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
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EYFP-Sec23A
Plasmid#66611PurposeYFP fusion of Sec23ADepositorInsertSec23A (SEC23A Human)
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_pFMD
Plasmid#160921PurposeFormate dehydrogenase (FMD) promoter module for episomal P. pastoris promoter shufflingDepositorInsertFormate dehydrogenase promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BbsI domesticatedPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-FLAG-ER-5
Plasmid#170622PurposeExpresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.DepositorInsertAPV-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_CoiC_CoiSA
Plasmid#208762PurposeTwo coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.DepositorInsertsCoiC
CoiSA
UseTagsExpressionBacterialMutationsingle nucleotide insertion (C70) at RBS of CoiCPromoterT7Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II 15
Plasmid#35177DepositorInsertRPBI (POLR2A Human)
UseTagsFLAGExpressionMammalianMutationContains the C-terminal repeats 16-52PromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEV_S
Plasmid#100266PurposepDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coliDepositorInsertsUseSynthetic Biology; Expression, pbr322TagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
(GGS)9_(+36GFP)-Cre.ape
Plasmid#71747Purpose+36-GFP-Cre fusion expression in E.ColiDepositorInsert(GGS)9_+36GFP-Cre
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II CC
Plasmid#35180DepositorInsertRPBI (POLR2A Human)
UseTagsFLAGExpressionMammalianMutationContains C-terminal domain repeats (1-22)X2PromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_pDHAS
Plasmid#160920PurposeDihydroxyacetone synthase (DHAS) promoter module for episomal P. pastoris promoter shufflingDepositorInsertDihydroxyacetone synthase promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BbsI domesticatedPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_pFDH1
Plasmid#160913PurposeFormate dehydrogenase (FDH1) promoter module for episomal P. pastoris promoter shufflingDepositorInsertformate dehydrogenase promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_pFLD1
Plasmid#160914PurposeFormaldehyde dehydrogenase (FLD1) promoter module for episomal P. pastoris promoter shufflingDepositorInsertFormaldehyde dehydrogenase promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only