We narrowed to 3,025 results for: PPL

1. pEasyG1_zeo

   Plasmid

   #184910

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPpT4- HTA1prom-TIR1 F74G -FLAG

   Plasmid

   #189728

   Purpose

   Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   HTA1

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. NAX_CAGGS_CAS9_m4_VPR_Puro

   Plasmid

   #167887

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SLC9A3R1_PDZ_2

   Plasmid

   #103933

   Purpose

   Protein expression and purification of SLC9A3R1 PDZ2 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   SLC9A3R2 (NHERF2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbdCas9S(-10AC12)_Psyn-sgRNArodA

   Plasmid

   #149661

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi rodA

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNArodA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30(-10AC12), Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PDLIM7_PDZ_1

   Plasmid

   #103940

   Purpose

   Protein expression and purification of ENIGMA PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PDLIM7 (PDLIM7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMG343

   Plasmid

   #176183

   Purpose

   preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   TPK2+ccdB dropout cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. NAX_CAGGS_CAS9_m4_VPR_EYFP

   Plasmid

   #167882

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

   Plasmid

   #149588

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi flaB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAflaB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSPYngK-hp

    Plasmid

    #48118

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of YngK
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1X-Nup93-mut

    Plasmid

    #87335

    Purpose

    To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP93 (NUP93 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

    Plasmid

    #149590

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAftsI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDSM-bc-Ptdh3-CAD-Ttdh1

    Plasmid

    #127727

    Purpose

    Yeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    cis-aconitate decarboxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Ptdh3

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC-KIT2

    Plasmid

    #118984

    Purpose

    Mutational dissection of the G rich region of the c-kit promoter

    Depositor

    Bjorn Hogberg

    Article

    Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

    Insert

    c-kit proto-oncogene {promoter} (KIT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutK2-SP-K1

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC-KIT4

    Plasmid

    #118986

    Purpose

    Mutational dissection of the G rich region of the c-kit promoter

    Depositor

    Bjorn Hogberg

    Article

    Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

    Insert

    c-kit proto-oncogene {promoter} (KIT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K2-SP-mutK1

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC-KIT6

    Plasmid

    #118988

    Purpose

    Mutational dissection of the G rich region of the c-kit promoter

    Depositor

    Bjorn Hogberg

    Article

    Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

    Insert

    c-kit proto-oncogene {promoter} (KIT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutK2-SP-mutK1

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSETa-Amrose v3

    Plasmid

    #79539

    Purpose

    pRSETa (Invitrogen) plasmid expressing Amrose variant 3

    Depositor

    Randolph Caldwell

    Article

    Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

    Insert

    Amrose v3
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

    Plasmid

    #149594

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BB1_34_RPS25Att

    Plasmid

    #98526

    Purpose

    BB1 with insert: RPS25Att

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    RPS25Att
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTFP1-Tubulin-6

    Plasmid

    #55512

    Purpose

    Localization: Microtubules, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only