Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 653 results for: PPL

Showing: 1 - 20 of 653 results

  1. PhD Applications After COVID

    Type
    Blog Post
    ...previous nosy question! The Application Process Broadly speaking, the PhD application process tends to go something...steps in the application process and usually occurs in the few months leading up to applications opening. ...program. Most applications will recommend that you name two professors in your application that you intend...to continue the application process through a campus visit, a weekend where applications can get to know...strengthen your application and help you decide if a program is a good fit for you. The application process looks...if you’re anything like me, the process of even applying to one of these programs is almost as daunting...be, at times, very overwhelming. As someone who applied to PhD programs in Biology around the United States...

  3. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...their use in other clinical applications. In the last decade, viral applications in cancer treatment and ...s unpack the myriad number of emerging viral applications and give you some recommendations for putting...putting viruses to work. Viral applications Viruses work by infecting a host cell (the target of viral infection...expressed. It’s important to note that for all the applications we discuss, there is more than one type of virus...retrovirus, AAV, etc.). When you’ve decided on an application, our Viruses as Biological Tools blog can help...viruses and pick one for your experiment. Research applications Gene expression control Researchers are always...becoming increasingly popular tools with many applications. These libraries include sgRNAs targeting the...

  4. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...have been developed for different scientific applications. These PCR methods all use the same basic PCR...quantified by qPCR. qPCR is used in a variety of applications including gene expression profiling, studying...

  5. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...recent advancements and offer commentary on their application to neuroscience research. What is CRISPR-based...accessible to neuroscientists, not all neuroscience applications require such optimized tools. This is elegantly...study neuronal differentiation, it could also be applied to the differentiation of other CNS cell types,...necessitates such molecular gymnastics for sensitive applications such as CRISPR screening. The plasmids used ...used by the Qi Lab for this particular application can be found here.  Why CRISPRa? Although cDNA overexpression...gene activation in select in vitro and in vivo applications, the tools described above make CRISPRa in the...relatively easy and accessible. There are also other applications for which CRISPRa is well-suited, including ...

  6. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...blog post, we're going to focus on PCR-based applications (qPCR and ddPCR.) qPCR Quantitative/real-time...range of RQ values as noted above.   In both applications, results can be affected by DNA quality and ... triplicates. ddPCR is an extremely accurate application, even in areas of the genome that are difficult...your variant options, you’re likely to find an application that matches your needs. Happy quantifying!  ...QuantificationPolymerase Chain Reaction Overview and Applications Resources on Addgene.org Protocol: Polymerase...

  7. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...and applications.    Plant applications We outlined many synthetic biology MoClo applications, but...- and multi-gene constructs for a variety of applications. MoClo tools can be used in bacterial and cell-free...and the kits Addgene offers to kickstart these applications, no matter what your model organism is. MoClo...this blog, we will highlight some common MoClo applications and kits. Keep in mind that this technology ... it, MoClo might help you achieve it! MoClo applications and specialized tools Synthetic biology in bacteria...and multi-TU constructs for synthetic biology applications in bacteria or cell-free systems. The approach...assembly can occur. It is quite extensive with a supplemental add-on kit and is compatible with E. coli and...

  8. Antibody Guide

    Type
    Collection
    ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...of other antibodies. This has useful research applications, and is covered in the following Isotypes section...signaling molecules and are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments... molecules that can be distinguished in your application. The indirect detection method uses an unconjugated...

  9. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...The amount of DNA that you cut depends on your application. A diagnostic digest typically involves ∼500 ...volume usually varies from 10-50 µL depending on application and is largely determined by the volume of DNA...manufacturer’s instructions. Pro-Tip Depending on the application and the amount of DNA in the reaction, incubation...you will be using the digested DNA for another application (such as a digestion with another enzyme in a...

  10. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...therapeutic applications. Despite the ethical controversies surrounding non-research applications, it’s clear...just as useful (if not more so) for research applications, eclipsing past genome engineering technologies...for CRISPR genomic editing. Genome Engineering Applications In 2012, Jinek et al. first demonstrated that...various species, as well as the development of new applications. The first CRISPR papers described two main ...hand, if a repair template with homology arms was supplied, the break could be repaired according to this...RNA, making it a very flexible system. Other Applications Scientists have also used the targeting capability...Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic disease, with the...

  11. All Antibodies

    Type
    Collection
    ...homology with the antigen. Addgene supplies a list of recommended applications based on our in-house testing...antibodies. These monoclonal antibodies undergo application-specific validation and quality control by Addgene...with full experimental details in the Antibody Applications section of our product pages. We also include... an antibody’s use is not recommended for an application or species. We currently assess western blot,...scientists to further develop and refine these application lists. The plasmids we use to produce antibodies...Source Species Isotype Reactivity Recommended Applications PI Return to Top Do you have suggestions for...

  12. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...and valproic acid supplementation During the 24–144 h post-transfection, supplement the flask with 4% ...antibody can be purified for use in a variety of applications. Sharing speeds science. We believe that sharing...culture volume. At 72-96 h post-transfection, supplement the flask with 3.75 mM valproic acid. Example...

  13. CRISPR Guide

    Type
    Collection
    ... to various Cas enzymes have extended CRISPR applications to increasingly complex functions, including...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR... Cas12a, which are available for specialized applications. Check out our list of additional Cas proteins...genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using...to help you select an optimized gRNA for your application (see: gRNA Design Software ). In addition to ...edits, they can be used for virtually any CRISPR application. Cas9 Orthologs While SpCas9 is the most commonly...require longer homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide...

  14. Antibodies

    Type
    Collection
    ...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

  15. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...controller Microcentrifuge Electrophoresis chamber Power supply Rocking platform Fume hood Metal spatula Razor ...the electrophoresis chamber and attach to a power supply. Run the gel at 150 V for 1 h . Pro-Tip If the ...“smile”, reduce the voltage. Turn off the power supply and unplug the electrophoresis chamber. Remove ...

  16. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England Biolabs offers non-toxic...Single constructs are compatible with multiple applications (different fluorophores, biotin, bead purification...MCP-tag Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and CLIP...purchasing coenzyme A disodium salt from a commercial supplier and conjugating it to the label of their choice...

  17. Microbiology Resources

    Type
    Collection
    ...our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology... cloning and expression systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors...Densmore Lab EcoFlex MoClo : Modular cloning for applications like recombinant protein purification and cell-free...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

  18. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Bacterial Expression mApple 568 592 37 6.5 30 min Monomer mApple-N1 - Mammalian Expression mApple-C1 - Mammalian...Mammalian Expression mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-...and emission maxima (Stokes shift), useful for applications like multicolor imaging with better spectral...

  19. Luciferase Plasmid Collection

    Type
    Collection
    ...advantages and disadvantages depending on the application, and different luciferases require different ... course experiments, but is too dim for some applications. NanoLuc® is small (19 kDa), very bright, and... available, making it ideal for a variety of applications. To learn more and plan your experiment, check...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored version...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...tTA-Advanced Takeshi Imai Other Tet Applications Explore some other popular applications of tetracycline systems ...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated...
Showing: 1 - 20 of 653 results