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We narrowed to 14 results for: PPL

Showing: 1 - 14 of 14 results

  1. Antibody Guide

    Type
    Guide
    ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...of other antibodies. This has useful research applications, and is covered in the following Isotypes section...signaling molecules and are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments... molecules that can be distinguished in your application. The indirect detection method uses an unconjugated...

  2. CRISPR Guide

    Type
    Guide
    ... to various Cas enzymes have extended CRISPR applications to increasingly complex functions, including...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR... Cas12a, which are available for specialized applications. Check out our list of additional Cas proteins...genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using...to help you select an optimized gRNA for your application (see: Plan Your Experiment ). In addition to ...edits, they can be used for virtually any CRISPR application. Cas9 Orthologs While SpCas9 is the most commonly...require longer homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ...library. Apply pooled library to cells. DIVERGE: Positive and negative screens POSITIVE screen: Apply library...control sample (no selection) In parallel, apply library Apply selection; most cells will survive Perform..., semi-random sequences that can be used for applications like lineage tracing or parsing the effects ... guessed from their name, pooled libraries are supplied as a mixed population of plasmids in a single ...library Apply selection Most cells die or do not pass selection (in the case of a reporter) Sort “winning...genes involved in the selection NEGATIVE screen: Apply library Perform Next Generation Sequencing (NGS)...

  4. Plan Your Experiment

    Type
    Guide
    ...Cas9 in mammalian cells, many of these principles apply to using CRISPR in other organisms. First, consider...may not be a perfect plasmid for your specific application, and in such cases, it may be necessary to customize...reagent to suit your needs. Genetic Manipulation Application Cas9 gRNA Additional Considerations Knockout ...method Expression System Components of System Application Mammalian expression vector Cas enzyme promoter...gene you target will depend on your specific application. For example: To activate or repress a target...validate your edit will depend upon your specific application. However, there are several common ways to verify...

  5. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...to be used as viral vectors for gene therapy applications. This guide contains many viral vector-specific...vector production, the Rep and Cap regions are supplied in trans , via a different plasmid. In order to...preferred over standard rAAV vectors for in vivo applications. Other Engineered Variants Hybrid capsids Hybrid...Vectors rAAV vectors are valuable in diverse applications due to their unique properties, including their...biomedical fields. Gene Therapy One of the major applications of rAAV vectors is in the field of gene therapy...transgenes, enabling the development of further AAV applications. Read our blog post on split AAVs for more information...

  6. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...retroviral vectors are commonly used in gene therapy applications to more specifically direct delivery of gene...lentiviral vectors, but a similar method can be applied when using gamma-retroviral vectors. Gene Therapy...vectors are a popular choice in gene therapy applications. There are many approved gamma-retroviral gene...cell (CAR-T) therapy is a particularly popular application of gamma-retroviral vectors. Gamma-retroviral...Gammaretroviral vectors: Biology, technology and application. Viruses , 3 (6), 677–713. https://doi.org/10.3390...

  7. Adenovirus Guide

    Type
    Guide
    ..., which has limited their use in therapeutic applications. However, this has also made them the most widely...adenoviral genes have been removed. These genes are now supplied in trans , via different plasmids or the packaging...for viral replication and therefore needs to be supplied through other means. In order to produce rAdV ...Adenovirus-mediated gene delivery: potential applications for gene and cell-based therapies in the new... . Journal of Genetic Syndromes & Gene Therapy, Suppl 5 , 001. https://doi.org/10.4172/2157-7412.s5-001...

  8. Addgene's eBook Collection

    Type
    Guide
    ...contains an introduction to each vector and its applications, tips for using vectors in your experiments,...your transferable skills, and tips on the job application process. Close About Overlay Select CRISPR 101...introductions and helpful tips for antibody-based applications, from flow to IHC! Close About Overlay 0 of ...

  9. Optogenetics Guide

    Type
    Guide
    ...the light source. Optogenetics has been broadly applied to study the physiology of the brain and nervous...conformation/dimerization. In addition to the applications described above, both LOV and Dronpa can control..., Yizhar O, Deisseroth K. 2011. Principles for applying optogenetic tools derived from direct comparative...

  10. Chemogenetics Guide

    Type
    Guide
    ...neurons) that can be turned on or off by the application of a small molecule ligand. The ideal chemogenetic...chemogenetics for research and potential clinical applications. Science. 364(6436):eaav5282. PMID: 30872534...

  11. Lentiviral Vector Guide

    Type
    Guide
    ...random and difficult to predict. In therapeutic applications, like chimeric antigen receptor T cell (CAR-...Pseudotyped lentiviral vectors are common in clinical applications, like gene therapy, as you can more specifically...

  12. Educational Resources

    Type
    Guide
    ...written and video protocols for a wide range of applications in molecular biology, plasmid cloning, and virus...

  13. Science Guides

    Type
    Guide
    ...specific binding to other proteins. Antibody-based applications, which often use antibodies conjugated to a ...

  14. Molecular Biology Reference

    Type
    Guide
    ...species and can be useful for a diverse set of applications - Plasmids can drive gene expression in a wide...
Showing: 1 - 14 of 14 results