We narrowed to 20 results for: PPL

...The amount of DNA that you cut depends on your application. A diagnostic digest typically involves ∼500 ...volume usually varies from 10-50 µL depending on application and is largely determined by the volume of DNA...manufacturer’s instructions. Pro-Tip Depending on the application and the amount of DNA in the reaction, incubation...you will be using the digested DNA for another application (such as a digestion with another enzyme in a...

...and valproic acid supplementation During the 24–144 h post-transfection, supplement the flask with 4% ...antibody can be purified for use in a variety of applications. Sharing speeds science. We believe that sharing...culture volume. At 72-96 h post-transfection, supplement the flask with 3.75 mM valproic acid. Example...

...controller Microcentrifuge Electrophoresis chamber Power supply Rocking platform Fume hood Metal spatula Razor ...the electrophoresis chamber and attach to a power supply. Run the gel at 150 V for 1 h . Pro-Tip If the ...“smile”, reduce the voltage. Turn off the power supply and unplug the electrophoresis chamber. Remove ...

...GenHunter: #Q401 FuGENE® 6 Transfection Reagent Roche Applied Biosciences: #11814443001 OPTI-MEM® serum-free ...lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen . Moffat...Terms of Use , Addgene’s Terms of Purchase , and applicable Material Transfer Agreements between the party...

...protocols that you can use for a wide range of applications, with videos for select protocols in the right-hand...preparation Antibodies Protocols for common antibody applications. Name Description Link to Video Transfection...

... reaction with both. MgCl 2 acts to supplement the amount supplied by the buffer stock, where it is known...

...column in a new 50 mL LoBind collection tube. Slowly apply the sample to the center of the compact resin bed...exchange and concentration with an Amicon column Apply 15 mL of PBS to the reservoir of a Amicon Ultra-...

...Spectrophotometer Heat block Mini gel tank chamber Power supply iBlot 2 Gel Transfer Device Roller Spatula Platform...tank and plug the electrode cords into the power supply. Run the gel at 100 V for 10–15 min or until the...

...Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129 ddPCR Supermix for Probes ... the BSC to be used for droplet generation are supplied with sufficient pipette tips and reagent reservoirs...

... not affect the quality of RNA or downstream applications. To improve yield of RNA, instead of incubating...

...according to speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed ...

... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered...

...working in the lab. Do not eat, drink, chew gum, or apply makeup in the laboratory. Before you start your ...

... take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture to induce membrane permeability...

...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your...

...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...

...the plasmid DNA. Either spin to pellet the DNA or apply the solution to a column that will bind the now ...

...lower dilutions depending on your downstream applications. If you’ve titered your virus beforehand, you...

...sufficient quantities of library for experimental applications. Repeated amplifications should be avoided as...

...Safety Cabinet (BSC). Make sure that the BSC is supplied with sufficient pipette tips. Pre-warm the 96-...