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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 20 results for: PPL

Showing: 1 - 20 of 20 results

  1. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...The amount of DNA that you cut depends on your application. A diagnostic digest typically involves ∼500 ...volume usually varies from 10-50 µL depending on application and is largely determined by the volume of DNA...manufacturer’s instructions. Pro-Tip Depending on the application and the amount of DNA in the reaction, incubation...you will be using the digested DNA for another application (such as a digestion with another enzyme in a...

  2. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...and valproic acid supplementation During the 24–144 h post-transfection, supplement the flask with 4% ...antibody can be purified for use in a variety of applications. Sharing speeds science. We believe that sharing...culture volume. At 72-96 h post-transfection, supplement the flask with 3.75 mM valproic acid. Example...

  3. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...controller Microcentrifuge Electrophoresis chamber Power supply Rocking platform Fume hood Metal spatula Razor ...the electrophoresis chamber and attach to a power supply. Run the gel at 150 V for 1 h . Pro-Tip If the ...“smile”, reduce the voltage. Turn off the power supply and unplug the electrophoresis chamber. Remove ...

  4. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...GenHunter: #Q401 FuGENE® 6 Transfection Reagent Roche Applied Biosciences: #11814443001 OPTI-MEM® serum-free ...lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen . Moffat...Terms of Use , Addgene’s Terms of Purchase , and applicable Material Transfer Agreements between the party...

  8. Western Blot

    Type
    Protocol
    ...Spectrophotometer Heat block Mini gel tank chamber Power supply iBlot 2 Gel Transfer Device Roller Spatula Platform...tank and plug the electrode cords into the power supply. Run the gel at 100 V for 10–15 min or until the...

  9. AAV ddPCR Titration

    Type
    Protocol
    ...Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129 ddPCR Supermix for Probes ... the BSC to be used for droplet generation are supplied with sufficient pipette tips and reagent reservoirs...

  10. Kit Free RNA Extraction

    Type
    Protocol
    ... not affect the quality of RNA or downstream applications. To improve yield of RNA, instead of incubating...

  11. Centrifugation

    Type
    Protocol
    ...according to speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed ...

  12. Fluorescence Titering Assay

    Type
    Protocol
    ... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered...

  15. Immunocytochemistry

    Type
    Protocol
    ...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your...

  16. Lentivirus Production

    Type
    Protocol
    ...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...

  19. CRISPR Library Amplification

    Type
    Protocol
    ...sufficient quantities of library for experimental applications. Repeated amplifications should be avoided as...

  20. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Safety Cabinet (BSC). Make sure that the BSC is supplied with sufficient pipette tips. Pre-warm the 96-...
Showing: 1 - 20 of 20 results