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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

Plasmid

#218864

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8s

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

Plasmid

#78509

Purpose

Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

CD45R0Ex (PTPRC Human)

Tags

His10 and SNAPf

Expression

Insect

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD-E12

Plasmid

#25990

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD-E12 Heterodimer Fusion (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD-MyoD

Plasmid

#25992

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD-MyoD homodimer (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only
pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

Plasmid

#218867

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8f

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-mCherry

Plasmid

#35508

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

Plasmid

#218865

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8s

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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R

Plasmid

#167170

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-R
ATP1B1

Expression

Bacterial and Insect

Promoter

P10 and PH

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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S

Plasmid

#167172

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-S
ATP1B1

Expression

Bacterial and Insect

Promoter

P10 and PH

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ADAP1

Plasmid

#163905

Purpose

Full length mouse ADAP1 for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)

Plasmid

#226884

Purpose

Expresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cells

Depositor

Ning Zheng

Article

Wisedchaisri et al Cell. 2019 Aug 8;178(4):993-1003.e12. do

Insert

NaVAb

Tags

MBP

Expression

Insect

Mutation

R4A, N49K, L109A, M116V

Promoter

Polyhedrin

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-D3-ASK1

Plasmid

#226619

Purpose

Co-expression of D3 and ASK1 complex in insect cells

Depositor

Ning Zheng

Article

Shabek et al Nature. 2018 Nov;563(7733):652-656. doi:

Inserts

D3
ASK1 (SKP1 Mustard Weed)

Tags

His tag followed with three copies of Msyb

Expression

Insect

Mutation

The flexible loop in D3 (Q478-D515) can be remove…

Promoter

Polyhedrin

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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KIF13B

Plasmid

#163907

Purpose

Full length mouse KIF13B for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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KIF13B 1-584

Plasmid

#163908

Purpose

Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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TBRG4 gRNA (BRDN0001162276)

Plasmid

#77703

Purpose

3rd generation lentiviral gRNA plasmid targeting human TBRG4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TBRG4 (TBRG4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TBRG4 gRNA (BRDN0001162363)

Plasmid

#77704

Purpose

3rd generation lentiviral gRNA plasmid targeting human TBRG4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TBRG4 (TBRG4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TBRG4 gRNA (BRDN0001146623)

Plasmid

#77705

Purpose

3rd generation lentiviral gRNA plasmid targeting human TBRG4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TBRG4 (TBRG4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAcBac1-BacMam-4x-PyOtR

Plasmid

#217364

Purpose

Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

4 copies of pyrrolysyl-tRNA mutant PyOtR

Use

Baculoviral

Expression

Insect and Mammalian

Promoter

U6

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.MTF2

Plasmid

#125169

Purpose

Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

MTF2 (MTF2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.PHF1

Plasmid

#125167

Purpose

Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

PHF1 (PHF1 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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