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We narrowed to 5,144 results for: PEP

Showing: 1701 - 1720 of 5144 results

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pAGM9121_pPMP20

Plasmid

#160915

Purpose

Peroxisomal glutathione oxidase (PMP20) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Peroxisomal glutathione oxidase promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pDAS1

Plasmid

#160916

Purpose

Dihydroxyacetone synthase 1 (DAS1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Dihydroxyacetone synthase 1 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pDAS2

Plasmid

#160917

Purpose

Dihydroxyacetone synthase 2 (DAS2) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Dihydroxyacetone synthase 2 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pALD4

Plasmid

#160919

Purpose

Mitochondrial aldehyde dehydrogenase (ALD4) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Mitochondrial aldehyde dehydrogenase promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pAOX1

Plasmid

#160912

Purpose

Alcohol oxidase 1 (AOX1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Alcohol oxidase 1 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II DC

Plasmid

#35181

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C-terminal domain repeats 1-22 and 31-52

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-IL2sp-6His-Asprosin plasmid

Plasmid

#190683

Purpose

Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

Depositor

Atul Chopra

Article

Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

Insert

IL2-6xHis-Asprosin

Use

Tags

IL2 signal peptide, 6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pECFP-Sec23A

Plasmid

#66610

Purpose

CFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
EC.SurA.pTYB1.a21-281&390-428.wt

Plasmid

#12607

Depositor

David McKay

Article

Bitto et al J Biol Chem. 2003 Dec 5. 278(49):49316-2

Insert

Escherichia coli surA delta PPIase domain 2

Use

Tags

intein

Expression

Bacterial

Mutation

residues 282-389 deleted

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y819W

Plasmid

#1307

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y819W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 819 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARFGEF2_Sec7

Plasmid

#109924

Purpose

Protein expression and purification of ARFGEF2_Sec7

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ARFGEF2_Sec7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pVP65KR-gb-SacB(-)

Plasmid

#171646

Purpose

Equipped with an autoinducible promoter. Possible sucrose selection. Works in a general host and in a general medium. Real-time monitoring of the protein production. 8xHis at the N-terminus.

Depositor

Young Kee Chae

Article

Chae et al Protein Pept Lett. 2021;28(12):1398-1407

Insert

MBP-mCherry-8xHis-SacB(-)

Use

Tags

8xHis

Expression

Mutation

internal HindIII site in the SacB gene was remove…

Promoter

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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H2B-MiniR-mEm

Plasmid

#181990

Purpose

MiniR genetically encoded tag on H2B-mEmerald to be used with MiniE labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

H2B-MiniR-mEm

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJZ002

Plasmid

#120231

Purpose

CRISPR-cas9 targeting in E. coli with ampicillin resistance

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-Cre.ape

Plasmid

#71749

Purpose

U+36GFP-Cre fusion expression in E. Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)-Cre

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_EGFR_Recep-L

Plasmid

#109920

Purpose

Protein expression and purification of EGFR_Recep-L

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EGFR_Recep-L

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX2TK-YAP-WW1

Plasmid

#104305

Purpose

bacterial expression of WW domain 1 of YAP; binds PPxY where Y is not phosphorylated

Depositor

Marius Sudol

Insert

YAP WW1 domain (YAP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

WW domain 1

Promoter

tac

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_TLN1_IRS

Plasmid

#109938

Purpose

Protein expression and purification of TLN1_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TLN1_IRS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Aurein1.2_(+36GFP)-BirA

Plasmid

#71752

Purpose

Aurein1.2+36GFP-BirA fusion for E.Coli expression

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2+36GFP-BirA

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_TRIP6_LIM

Plasmid

#109941

Purpose

Protein expression and purification of TRIP6_LIM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TRIP6_LIM

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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