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pLP-PH16_RS09790-pcepA-himar-chlorR

Plasmid

#238494

Purpose

Landing Pad with BenM/PH16_RS09790, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

BenM/PH16_RS09790

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLP-PH16_RS09790-pcepA-himar-chlorR

Plasmid

#238494

Purpose

Landing Pad with BenM/PH16_RS09790, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

BenM/PH16_RS09790

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLP-ParaBAD-pcepA-himar-chlorR

Plasmid

#238491

Purpose

Landing Pad with AraC/ParaBAD, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

AraC/ParaBAD

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLP-PH16_RS08125-pcepA-himar-chlorR

Plasmid

#238493

Purpose

Landing Pad with NahR/PH16_RS08125, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

NahR/PH16_RS08125

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLP-ParaBAD-pcepA-himar-chlorR

Plasmid

#238491

Purpose

Landing Pad with AraC/ParaBAD, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

AraC/ParaBAD

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLP-PH16_RS08125-pcepA-himar-chlorR

Plasmid

#238493

Purpose

Landing Pad with NahR/PH16_RS08125, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

NahR/PH16_RS08125

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
P-d22bp

Plasmid

#235657

Purpose

B. thetaiotaomicron fusA2 expression reporter lacking 22bp Cur binding site

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides thetaiotaomicron fusA2 promoter lacking the 22bp Cur binding site

Mutation

The B. thetaiotaomicron fusA2 promoter lacking &q…

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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P-d22bp

Plasmid

#235657

Purpose

B. thetaiotaomicron fusA2 expression reporter lacking 22bp Cur binding site

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides thetaiotaomicron fusA2 promoter lacking the 22bp Cur binding site

Mutation

The B. thetaiotaomicron fusA2 promoter lacking &q…

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT2-μ4

Plasmid

#198176

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-4 μ4

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT2-μ4

Plasmid

#198176

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-4 μ4

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7-AP-1 σ1B

Plasmid

#198335

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1B

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Expresses a LELQMNRRY nonapeptide after the last …

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7-AP-1 σ1B

Plasmid

#198335

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1B

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Expresses a LELQMNRRY nonapeptide after the last …

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7-AP-1 σ1A

Plasmid

#198334

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1A

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Expresses a LELQMNRRY nonapeptide after the last …

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7-AP-1 σ1A

Plasmid

#198334

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1A

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Expresses a LELQMNRRY nonapeptide after the last …

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT2-μ2

Plasmid

#198173

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-2 μ2

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT2-μ2

Plasmid

#198173

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-2 μ2

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C2-tepsin

Plasmid

#196707

Purpose

Expresses GFP-tepsin in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

tepsin (TEPSIN Human)

Tags

EGFP

Expression

Mammalian

Mutation

silent substitution in codon 199 to eliminate int…

Promoter

CMV

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-tepsin

Plasmid

#196707

Purpose

Expresses GFP-tepsin in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

tepsin (TEPSIN Human)

Tags

EGFP

Expression

Mammalian

Mutation

silent substitution in codon 199 to eliminate int…

Promoter

CMV

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB202

Plasmid

#169113

Purpose

TESEC expression vector for mEGFP

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

mEGFP

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB202

Plasmid

#169113

Purpose

TESEC expression vector for mEGFP

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

mEGFP

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge