We narrowed to 4,338 results for: BFP

1. LV anti-FITC-LaG16-sNRR-T2A-BFP

   Plasmid

   #194930

   Purpose

   LV construct encoding sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

   Plasmid

   #216664

   Purpose

   Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance

   Depositor

   Leonardo Morsut

   Inserts

   ETV2 (Etv2 Mouse)
   BFP
   HygromycinR
   

   Use

   Lentiviral

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

   Plasmid

   #193968

   Purpose

   Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   hPol eta (POLH Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

   Plasmid

   #226009

   Purpose

   DjCas13d guide RNA cloning backbone (lenti)

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRhobin2-SEC61B-IRES-NLS-mTagBFP-2xNLS

   Plasmid

   #239024

   Purpose

   Co-overexpression of Rhobin2-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

   Depositor

   Bo Huang, William DeGrado

   Article

   Chen et al bioRxiv 2025.06.24.661379

   Insert

   Rhobin2-SEC61B (SEC61B Synthetic, Human)
   

   Tags

   TagBFP

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-UCOE-dCas9-BFP-Zim3-KRAB

   Plasmid

   #196716

   Purpose

   CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-TagBFP-KRAB(ZIM3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(46))-PGKpuroBFP-W

   Plasmid

   #200504

   Purpose

   Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   SMARCA4(43), SMARCA2(46) (SMARCA2, SMARCA4 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. EK0208 CMV-TO-BFPmut-stop-t70587 (FLP-IN)

   Plasmid

   #191138

   Purpose

   Expression of Trigger 1 in 3' UTR of an inactive BFP in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   t70587
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

   Plasmid

   #236231

   Purpose

   AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
   

   Use

   AAV

   Tags

   mTagBFP2

   Promoter

   human Synapsin 1

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)

    Plasmid

    #191146

    Purpose

    Expression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    Bdnf.t1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2

    Plasmid

    #205160

    Purpose

    express β-ACTIN-3′UTR-8xMS2

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    β-ACTIN-3′UTR-24xMS2
    

    Tags

    tagBFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCF525-EF1a-Hygro-P2A-mTagBFP2-lenti

    Plasmid

    #115797

    Purpose

    Expresses mTagBFP in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    mTagBFP
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Promoter

    EF-1a promoter

    Available Since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-3xNLS-mTagBFP2-Cre (JDW 918)

    Plasmid

    #224517

    Purpose

    A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xNLS-mTagBFP2-Cre
    

    Use

    Cre/Lox

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

    Plasmid

    #226011

    Purpose

    CasRx guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP

    Plasmid

    #202824

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 - two

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

    Plasmid

    #128755

    Purpose

    Expresses CYRANO gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    CYRANO sgRNA (OIP5-AS1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcg 2.0-BFP-circ-arRNA backbone

    Plasmid

    #213965

    Purpose

    pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-AID-PcrA-UGI-T2A-mTagBFP2

    Plasmid

    #221224

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA-UGI-T2A-mTagBFP2
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-anti-GFP/BFP(LaG2) PAGER(FL)

    Plasmid

    #230022

    Purpose

    Expresses anti-GFP/BFP PAGER(FL) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    pAAV-MT3-LaG2-TEVcs-ALFA-hM4DxcpGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

    Plasmid

    #219687

    Purpose

    Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

    Depositor

    Jennifer Nemhauser

    Insert

    T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
    

    Expression

    Plant

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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