We narrowed to 32,518 results for: Eng

1. pBoBi-hTOMM20-C-PEN2

   Plasmid

   #183647

   Purpose

   Lentiviral expression of a mitochondrion-residential PEN2.

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 and TOMM20 (PSENEN, TOMM20 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLL1 sgRNA(MS2)-MS2-AIDmono

   Plasmid

   #112127

   Purpose

   empty sgRNA cloning vector with MS2-AIDmono

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AID mono mutant (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, H130A/R131E…

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL3 sgRNA(MS2)-MS2-AID3f

   Plasmid

   #112129

   Purpose

   empty sgRNA cloning vector with MS2-AID3f

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AIDmono 3f mutant (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, F115Y/C116F…

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL2 sgRNA(MS2)-MS2-AID3c

   Plasmid

   #112128

   Purpose

   empty sgRNA cloning vector with MS2-AID3c

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AID mono 3c mutant (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, F115Y/C116F…

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pvalb-2A-Dre Flp-in replacement vector

   Plasmid

   #61571

   Purpose

   Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Pvalb-2A-Dre (Pvalb Mouse, D6 Bacteriophage)
   

   Use

   Recombinase-mediated cassette exchange using flp …

   Available Since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-TetO-hSOX2-C17

   Plasmid

   #206385

   Purpose

   Expresses human SOX2-C17 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

   Insert

   SOX2-C17 chimera (SOX2, SOX17 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.3-hAP1 1-419

   Plasmid

   #183644

   Purpose

   To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   ATP6AP1 (ATP6AP1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   truncated ATP6AP1 mutant; contains only amino aci…

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBoBi-hAP1 Δ420-440

   Plasmid

   #183642

   Purpose

   To (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expression

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   ATP6AP1 (ATP6AP1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   truncated ATP6AP1 mutant lacking the transmembran…

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBoBi-hSEC61B-C-PEN2

   Plasmid

   #183648

   Purpose

   Lentiviral expression of a ER-residential PEN2.

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 and SEC61B (PSENEN, SEC61B Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   WT

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYY166 pcDNA3.4-His-MBP-hAID

    Plasmid

    #231942

    Purpose

    Expression of His-MBP-AID fusion protein in mammalian cells

    Depositor

    Fei-Long Meng

    Article

    Wang et al Cell. 2023 May 11;186(10):2193-2207.e19.

    Insert

    AID (AICDA Human)
    

    Tags

    His-MBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. MOS

    Plasmid

    #64120

    Purpose

    A modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genes

    Depositor

    Linzhao Cheng

    Article

    Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

    Insert

    OCT4-2A-SOX2 (POU5F1, SOX2 Human)
    

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. MMK

    Plasmid

    #64121

    Purpose

    a modified episomal (EBNA1/OriP) vector expressing human Myc and KLF4 genes

    Depositor

    Linzhao Cheng

    Article

    Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

    Insert

    MYC-2A-KLF4 (MYC, KLF4 Human)
    

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pattB-LSL-AAVR-F2A-spCas9

    Plasmid

    #202459

    Purpose

    The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.

    Depositor

    Jan Carette

    Article

    Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d

    Insert

    AAVR (AU040320 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    mCherry fusion

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.3-hAP1 420-440

    Plasmid

    #183643

    Purpose

    To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    ATP6AP1 (ATP6AP1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    truncated ATP6AP1 mutant; contains only the trans…

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBoBi-hAP1-C-LAMP2 TM

    Plasmid

    #183645

    Purpose

    Lentiviral expression of a mutant of ATP6AP1 with its PEN2-interacting domain replaced with the transmembrane (TM) domain of LAMP2.

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    ATP6AP1 and LAMP2 (ATP6AP1, LAMP2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    ATP6AP1 transmembrane domain replaced by the tran…

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pShuttle-Flag-HA

    Plasmid

    #63681

    Purpose

    adenoviral shuttle vector containing N-terminal Flag tag

    Depositor

    Qingbo Xu, Lingfang Zeng

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dendra2-NS3

    Plasmid

    #238004

    Purpose

    Expresses NS3 in mammalian/mouse cell line

    Depositor

    Partha Mondal

    Insert

    NS3
    

    Tags

    Dendra2

    Expression

    Mammalian

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pANY4

    Plasmid

    #164897

    Purpose

    Two valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.

    Depositor

    Yingfeng An

    Insert

    ccdB cassette
    

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CoV2-Spike-D614G-Beta

    Plasmid

    #177962

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )
    

    Expression

    Mammalian

    Mutation

    K417N, E484K, N501Y, D614G

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CoV2-Spike-D614G-Epsilon

    Plasmid

    #177964

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G) (S )
    

    Expression

    Mammalian

    Mutation

    S13I, W152C, L452R, D614G

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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