We narrowed to 3,972 results for: gem

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pPML1 (GB0045)

Plasmid

#68164

Purpose

Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtML1 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-ColH-HylB

Plasmid

#248631

Purpose

expresses Myc-tagged ColH and His-tagged HylB as two separate proteins in E. coli

Depositor

Edwin Manuel

Publication

Avsharian et al Biomolecules. 2024 Nov 17;14(11):1458. d

Insert

Myc-tagged bacterial collagenase, ColH, and His-tagged hyaluronidase, HylB

Tags

His and Myc

Expression

Bacterial

Promoter

araBAD

Available since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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PVT1-luciferase

Plasmid

#113350

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PVT1-enhancer (PVT1 Human)

Use

Luciferase

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt1(D323A/D325A)

Plasmid

#86630

Purpose

Expresses eGFP-tagged human Akt1(D323A/D325A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Akt1(D323A/D325A) (AKT1 Human)

Expression

Mammalian

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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tFucci(CA)5pPBbsr2

Plasmid

#197351

Purpose

Encoding tFucci(CA)5.

Depositor

Michiyuki Matsuda

Publication

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tFucci(CA)5

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Mammalian

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Publication

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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px330-CD4

Plasmid

#136938

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

Depositor

Jeremy Stark

Publication

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting CD4 (CD4 Human)

Use

CRISPR

Expression

Mammalian

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFBD-Akt13C(S473D)

Plasmid

#86577

Purpose

Expresses cleavable human full-length Akt1(S473D) phosphomimetic

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130; S473D phos…

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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PVT1-luciferase

Plasmid

#113350

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PVT1-enhancer (PVT1 Human)

Use

Luciferase

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt1(D323A/D325A)

Plasmid

#86630

Purpose

Expresses eGFP-tagged human Akt1(D323A/D325A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Akt1(D323A/D325A) (AKT1 Human)

Expression

Mammalian

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Publication

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
px330-CD4

Plasmid

#136938

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

Depositor

Jeremy Stark

Publication

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting CD4 (CD4 Human)

Use

CRISPR

Expression

Mammalian

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFBD-Akt13C(S473D)

Plasmid

#86577

Purpose

Expresses cleavable human full-length Akt1(S473D) phosphomimetic

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130; S473D phos…

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pJC5-4-insulator-BREreporter-insulator

Plasmid

#171499

Purpose

injections to make Bmp reporter mouse transgenic line

Depositor

James Briscoe

Publication

Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Insulator BRE-MLP:H2B-Turqoise

Expression

Mammalian

Promoter

BRE-MLP

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only
pJC5-4-insulator-BREreporter-insulator

Plasmid

#171499

Purpose

injections to make Bmp reporter mouse transgenic line

Depositor

James Briscoe

Publication

Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Insulator BRE-MLP:H2B-Turqoise

Expression

Mammalian

Promoter

BRE-MLP

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only
pTU1

Plasmid

#72934

Purpose

Backbone vector with internal BsaI removed by site-directed mutagenesis

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Type

Empty backbone

Use

Synthetic Biology

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJRTCLCrVB1.3

Plasmid

#22844

Depositor

Niki Robertson

Publication

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

B DNA

Use

Cotton vigs vector

Expression

Bacterial

Mutation

1.3 unit B componet length inserted into pBlueScr…

Available since

March 16, 2010

Availability

Academic Institutions and Nonprofits only

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pTU1

Plasmid

#72934

Purpose

Backbone vector with internal BsaI removed by site-directed mutagenesis

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Type

Empty backbone

Use

Synthetic Biology

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJRTCLCrVB1.3

Plasmid

#22844

Depositor

Niki Robertson

Publication

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

B DNA

Use

Cotton vigs vector

Expression

Bacterial

Mutation

1.3 unit B componet length inserted into pBlueScr…

Available since

March 16, 2010

Availability

Academic Institutions and Nonprofits only

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pISH-En2

Plasmid

#84310

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for En2. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

En2 (En2 Mouse)

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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