We narrowed to 137,885 results for: Mal;

1. pAAV-SMVP-Cas9N-U6-gRNA P2

   Plasmid

   #80944

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK-flag-USP19 C506S

   Plasmid

   #78584

   Purpose

   Expression flag-USP19 C506S in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 C506S (USP19 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   mutate 506 cysteine residue to serine

   Promoter

   CMV

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

3. GWF297

   Plasmid

   #133612

   Purpose

   TET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFP

   Depositor

   Dustin Maly

   Article

   Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

   Insert

   TET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS2CP(V29I)-myc-T2A-tagRFP

   Plasmid

   #140260

   Purpose

   Expresses MS2CP(V29I) in mammalian cells

   Depositor

   Hirohide Saito

   Article

   Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

   Insert

   Coat protein of bacteriphage MS2
   

   Use

   Synthetic Biology

   Tags

   myc-T2A-tagRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXPR_212

   Plasmid

   #133457

   Purpose

   U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ef1a_NANOG_Ires_Puro

   Plasmid

   #18920

   Depositor

   Linzhao Cheng

   Article

   Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

   Insert

   NANOG (NANOG Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

8. px459 VRER

   Plasmid

   #101716

   Purpose

   sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E and T1337R)

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPD1029 ZF43x6-Compact(CMVMin)_EYFP

   Plasmid

   #138913

   Purpose

   EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a CMV minimal promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x6-Compact(CMVmin)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   ZF43x6-Compact(CMVmin)

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK-flag-USP19 1-1290

    Plasmid

    #78579

    Purpose

    Expression flag-USP19 1-1290 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 1-1290 (USP19 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutate 1291 residue to stop condon

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pRSF-1b-S14

    Plasmid

    #128596

    Purpose

    Expresses tagless 30S ribosomal subunit protein S14 in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S14 (rpsN E.coli)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. M-SPn-VP64

    Plasmid

    #48674

    Purpose

    Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Mutation

    nuclease-null

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pGH156_MS2-AID-Hygro

    Plasmid

    #85415

    Purpose

    lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AID and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. [Bla][IRES][GFP]

    Plasmid

    #97193

    Purpose

    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Inserts

    beta-Lactamase
    GFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMET7-GAG-HRAS

    Plasmid

    #80604

    Purpose

    Mammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    HIV-1 p55 GAG
    

    Expression

    Mammalian

    Promoter

    SRalpha

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. px330 VRER

    Plasmid

    #101729

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX-uORF-HA-birA*-STOP-IRES-Puro

    Plasmid

    #120558

    Purpose

    Expresses control HA-birA* in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    birA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-mPer2ER-pBABEpuro

    Plasmid

    #61430

    Purpose

    Express mPER2-ER* fusion protein

    Depositor

    Aziz Sancar

    Article

    Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

    Insert

    mPER2 (Per2 Mouse)
    

    Use

    Retroviral

    Tags

    Flag and mouse estrogen receptor ligand binding d…

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET-S9

    Plasmid

    #132948

    Purpose

    Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S9 (rpsI E.coli)
    

    Tags

    N-terminal 6xHis-tag

    Expression

    Bacterial

    Mutation

    E52C

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge