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pTC436

Plasmid

#91222

Purpose

protoplast vector expressing gRNA38 targeting barley MLO (with TREX2 expression)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting barley MLO

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTC437

Plasmid

#91219

Purpose

protoplast vector for targeted deletion of MLO gene in barley (CmYLCV promoter driving the gRNA array)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting MLO gene in barley

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-13b-1

Plasmid

#22762

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 13b-1 (mir-13b-1 Fly, primary transcript)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pRCIscript-crhr2_TAL1A

Plasmid

#44236

Depositor

Karl Clark, Stephen Ekker

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

crhr2 pTAL scaffold 1A

Use

Mrna transcription vector

Promoter

T3

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRCIscript-crhr2_TAL1B

Plasmid

#44237

Depositor

Karl Clark, Stephen Ekker

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

crhr2 pTAL scaffold 1B

Use

Mrna transcription vector

Promoter

T3

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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GoldyTALEN-cdh5 TAL1

Plasmid

#41833

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

cadherin 5 (cdh5 Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbmiR164e/h

Plasmid

#231156

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbmiR164e/h

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbmiR164e/h

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_SlMIR164b

Plasmid

#231152

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting SlMIR164b

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting SlMIR164b

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbATML1-1pro

Plasmid

#231151

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting NbATML1-1pro

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbSTM-5'UTR

Plasmid

#231150

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting NbSTM-5?UTR

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting NbSTM-5?UTR

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbPDS

Plasmid

#231146

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDS

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-2_NbPDS

Plasmid

#231145

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDS

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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AA369

Plasmid

#215987

Purpose

Fragmid fragment: (N' terminus) attenuate expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; u(S)ORF_v1.1; Stop; Start

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA370

Plasmid

#215988

Purpose

Fragmid fragment: (N' terminus) attenuate expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; u(S)ORF_v1.1; Stop; Start; BPNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-6-AmCyan-P2A-NptII-9

Plasmid

#197742

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AmCyan-P2A-NptII bicistronic CDS

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTC3cIfm

Plasmid

#193778

Purpose

Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

Frame-shifted CI

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGe1_J

Plasmid

#146688

Purpose

Insect Expression of DmGe1

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

DmGe1 (Ge-1 Fly)

Expression

Insect

Mutation

two non silent mutations Q194R and I1008T compare…

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R160a

Plasmid

#173756

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160a

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/R160a_sgRNA2/R160a

Use

CRISPR

Promoter

AtU6 &StU6

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R160b

Plasmid

#173757

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160b

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/R160b_sgRNA2/160b

Use

CRISPR

Promoter

AtU6 &StU6

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R390a

Plasmid

#173758

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390a

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/390a_sgRNA2/390a

Use

CRISPR

Promoter

AtU6 &StU6

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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