We narrowed to 284,823 results for: addgene

1. pCAG-Fab_8D3_2_L

   Plasmid

   #176076

   Purpose

   Express light chain of Fab_8D3_2 in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   Light chain of Fab_8D3_2
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. VPS35 shRNA2

   Plasmid

   #163859

   Purpose

   shRNA targeting the coding sequences of VPS35

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   VPS35 retromer complex component (VPS35 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiSAMPHv2

   Plasmid

   #167934

   Purpose

   All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lys-eMagA-EGFP

   Plasmid

   #162246

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   p18-eMagA-EGFP
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGH125_dCas9-Blast

   Plasmid

   #85417

   Purpose

   lentiviral expression vector for dCas9 with Blasticidin selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   dCas9 and Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PNCA-GFP

   Plasmid

   #67789

   Purpose

   Use together with pCMV-intron and VSV-G to package MMLV reporter virus

   Depositor

   Stephen Goff

   Article

   Ooi et al Epigenetics Chromatin. 2010 Sep 24;3(1):

   Insert

   MMLV-LTR-GFP
   

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

7. HLA_A2402

   Plasmid

   #165010

   Purpose

   Expression of HLA-A*24:02 cDNA

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   HLA-A*24:02 (HLA-A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWZL hygro 12S E1A

   Plasmid

   #18748

   Depositor

   Scott Lowe

   Article

   Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

   Insert

   12S E1A
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hsyn-GRAB_Ado1.0

   Plasmid

   #153285

   Purpose

   Express the adenosine sensor GRAB_Ado1.0 in neurons

   Depositor

   Yulong Li

   Article

   Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

   Insert

   Adenosine sensor GRAB_Ado1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ERmoxGFP

    Plasmid

    #68072

    Purpose

    mammalian expression of ER localized moxGFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Insert

    moxGFP
    

    Tags

    KDEL ER retention sequence and bovine prolactin s…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVCT-tTR-KRAB

    Plasmid

    #11643

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    CAG promoters, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHPRT-DRGFP

    Plasmid

    #26476

    Purpose

    DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting sequences

    Depositor

    Maria Jasin

    Article

    Pierce et al Genes Dev. 2001 Dec 15. 15(24):3237-42.

    Insert

    pHPRT-DRGFP
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCrlM

    Plasmid

    #122444

    Purpose

    Expresses myc tagged mouse reelin ORF in mammalian cells

    Depositor

    Tom Curran

    Article

    D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

    Insert

    reelin (Reln Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-puro-shNT

    Plasmid

    #109012

    Purpose

    short-hairpin knockdown of targeted gene

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting shRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6 promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ptet-RFP-shR-rtTA

    Plasmid

    #35625

    Purpose

    Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

    Depositor

    Howard Gu

    Article

    Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

    Type

    Empty backbone

    Use

    AAV and RNAi; Tet inducible

    Expression

    Mammalian

    Promoter

    Ptet

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. HTATIP (2OU2)

    Plasmid

    #33338

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HTATIP (KAT5 Human)
    

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 11, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

17. CAG-GFP/cre

    Plasmid

    #49054

    Purpose

    Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase

    Depositor

    Fred Gage

    Article

    Tashiro et al Nat Protoc. 2006;1(6):3049-55.

    Insert

    GFP/Cre
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. MXS_PGK::ZeoR-bGHpA

    Plasmid

    #62442

    Purpose

    MXS_chaining vector with PGK::ZeoR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Zeocin with PGK Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW-eGFP

    Plasmid

    #162823

    Purpose

    Doxycycline-inducible overexpression of eGFP

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V163A

    Promoter

    Tet ON

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFLAG-SURF4

    Plasmid

    #170680

    Purpose

    Expresses FLAG-tagged SURF4 in mammalian cells.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    SURF4
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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