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pEBTet-SNAP-POC5

Plasmid

#136786

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-POC5 (POC5 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-CaMKKbeta

Plasmid

#32453

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Tags

FLAG and GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-STIL

Plasmid

#136795

Purpose

Mammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-STIL (STIL Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3

Plasmid

#127128

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_RACK1

Plasmid

#127123

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA RACK1 (RACK1 Human)

Use

CRISPR

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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mt deltaN20 TFB2MdeltaC3

Plasmid

#205062

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 3 aa

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMTH PKC alpha

Plasmid

#8409

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C alpha (Prkca Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pEBTetBl-CLIP-STIL

Plasmid

#136844

Purpose

Mammalian expression of the centrosomal protein STIL N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-STIL (STIL Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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MKK2

Plasmid

#29580

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

MKK2 (MAP2K2 Human)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Constitutively active mutant; Starting Met remove…

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-Centrin3

Plasmid

#136806

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin3

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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Erk1

Plasmid

#29578

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Erk1 (MAPK3 Human)

Tags

FLAG, HIS6, and TEV

Expression

Bacterial

Mutation

K289N mutation present. Starting Met removed from…

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7b1

Plasmid

#14622

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b1 (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
KHBD00222

Plasmid

#34370

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1832 (CG1832 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA_DiCre_59.F2

Plasmid

#60207

Purpose

Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Synthetic, Bacteriophage P1, Human)

Tags

FKBP12 and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 19-59 of the humanized version of Cre…

Promoter

CMV

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLTR PKC beta II

Plasmid

#8417

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Rat Protein Kinase C beta II (Prkcb Rat)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7b2

Plasmid

#14623

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b2 (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
Lentiviral FLAG-CaMKKbeta

Plasmid

#32458

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Lentiviral

Tags

FLAG

Available Since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00233

Plasmid

#34377

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7803 (z Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00322

Plasmid

#34447

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8591 (CTCF Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pLTR PKC delta

Plasmid

#8419

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C delta (Prkcd Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only