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We narrowed to 4,839 results for: pcr

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Showing: 1741 - 1760 of 4839 results

  1. pJL259

    Plasmid
    #243698
    Purpose
    A piggyBac vector containing the endogenous NDUFB9 genomic locus, with its native 3' UTR replaced by the Actb 3' UTR, modified with silent mutations to allow for PCR-based analysis
    Depositor
    Jonathan Weissman
    Publication
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    NDUFB9 genomic locus, with its 3' UTR replaced by the ACTB 3' UTR (NDUFB9 Human)
    Expression
    Mammalian
    Available since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243698-plasmid-map-sequence-id-489323

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  2. pJL354

    Plasmid
    #243709
    Purpose
    A piggyBac vector containing the endogenous COX7A2 genomic locus, with its native 5' UTR replaced by the Actb 5' UTR, modified with silent mutations to allow for PCR-based analysis
    Depositor
    Jonathan Weissman
    Publication
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    COX7A2 genomic locus, with its 5' UTR replaced by the ACTB 5' UTR (COX7A2 Human)
    Expression
    Mammalian
    Available since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243709-plasmid-map-sequence-id-489299

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  3. pTE5423_p3WJdB-glcO60

    Plasmid
    #221194
    Purpose
    DNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor system
    Depositor
    Tobias Erb
    Publication
    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0
    Insert
    T7 promoter-glcO60-3WJdB
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7 promoter with downstream glcO60 operator
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221194-plasmid-map-sequence-id-441878

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  5. pAct-GST-H3

    Plasmid
    #182842
    Purpose
    A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.
    Depositor
    Peter Harte
    Publication
    Tie et al Development. 2009 Sep;136(18):3131-41. d
    Insert
    GST-histone H3 fusion
    Tags
    GST
    Expression
    Insect
    Promoter
    Actin5C
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182842-plasmid-map-sequence-id-360059

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  6. PASTA-5_pUC19_IVT-ePa01

    Plasmid
    #247041
    Purpose
    Plasmid for in vitro transcription (IVT) of enhanced Pa01 (ePa01) mRNA. Use indicated PCR-primers for correction of T7-mutation and addition of poly-A-tail.
    Depositor
    Dimitrios Wagner
    Publication
    Kassing et al bioRxiv 2025.09.10.675267
    Insert
    ePa01 recombinase (large serine integrase, LSR) for IVT
    Expression
    Bacterial and Mammalian
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    247041-plasmid-map-sequence-id-500291

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  7. PASTA-1_pUC19_HDR-Bxb1-attG_TRAC_for-Cas12a

    Plasmid
    #247037
    Purpose
    Encodes HDR-template for Cas12a-mediated Knock-In of Bxb1-attG (landing pad) into TRAC gene (exon1). Template region to be PCR-amplified.
    Depositor
    Dimitrios Wagner
    Publication
    Kassing et al bioRxiv 2025.09.10.675267
    Insert
    Bxb1 bacterial attachment site (attB) = genomic attachment site (attG), flanked by human TRAC homology arms
    Expression
    Bacterial
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    247037-plasmid-map-sequence-id-499187

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  8. pNDGG039

    Plasmid
    #231339
    Purpose
    Terminator; BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
    Expression
    Bacterial
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231339-plasmid-map-sequence-id-472145

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  9. pNDGG038

    Plasmid
    #231338
    Purpose
    Terminator; BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
    Expression
    Bacterial
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231338-plasmid-map-sequence-id-472130

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  10. pNDGG037

    Plasmid
    #231337
    Purpose
    Terminator; BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
    Expression
    Bacterial
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231337-plasmid-map-sequence-id-472127

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  12. PASTA-3_pUC19_HDR-Pa01-attG_TRAC_for-Cas12a

    Plasmid
    #247039
    Purpose
    Encodes HDR-template for Cas12a-mediated Knock-In of size-reduced Pa01-attG (landing pad) into TRAC gene (exon1). Template region to be PCR-amplified.
    Depositor
    Dimitrios Wagner
    Publication
    Kassing et al bioRxiv 2025.09.10.675267
    Insert
    Pa01 bacterial attachment site (attB) = genomic attachment site (attG), flanked by human TRAC homology arms
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    247039-plasmid-map-sequence-id-499186

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  15. pGEM-2t

    Plasmid
    #159752
    Purpose
    CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    sgRNA
    Use
    CRISPR
    Available since
    Oct. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159752-plasmid-map-sequence-id-313824

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  16. hNTo1-qgRNA-pYJA5

    Plasmid
    #217781
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217781-plasmid-map-sequence-id-466019

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  17. hNTa1-qgRNA-pYJA5

    Plasmid
    #217779
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217779-plasmid-map-sequence-id-466021

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  18. hNTo2-qgRNA-pYJA5

    Plasmid
    #217782
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217782-plasmid-map-sequence-id-466020

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  20. hNTa2-qgRNA-pYJA5

    Plasmid
    #217780
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217780-plasmid-map-sequence-id-469426

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Showing: 1741 - 1760 of 4839 results