We narrowed to 6,477 results for: sas

1. pCMV-myc-ERK2-L4A-MEK1_fusion

   Plasmid

   #39197

   Depositor

   Melanie Cobb

   Article

   Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

   Insert

   ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   L4A = L33A, L37A, L40A, L42A

   Promoter

   CMV

   Available since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINB-hRNF216(511-784)

   Plasmid

   #171923

   Purpose

   Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.

   Depositor

   Bernhard Lechtenberg

   Article

   Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

   Insert

   E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
   

   Use

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   codon optimised for expression in E. coli

   Promoter

   T7

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-hDKK1-flag

   Plasmid

   #15494

   Depositor

   Sergei Sokol

   Article

   Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

   Insert

   DKK1 (DKK1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pFRT-DHX36iso1E335A

   Plasmid

   #159587

   Purpose

   Express N-ter FLAG HA tagged DHX36 isoform 1 with E335A mutation in mammalian cells

   Depositor

   Markus Hafner

   Article

   Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

   Insert

   DHX36 (DHX36 Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   E335A

   Promoter

   CMV

   Available since

   May 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHiUGE smFP-V5 donor (all-in-one) ORF1

   Plasmid

   #200387

   Purpose

   Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

   Insert

   spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pegCTNNB1 S45F

   Plasmid

   #169843

   Purpose

   pegRNA used for installation (via C•G-to-T•A) of the oncogenic S45F in Ctnnb1 in mouse liver.

   Depositor

   Wen Xue

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   pegCTNNB1 S45F (Ctnnb1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-cfGFP

   Plasmid

   #200773

   Purpose

   Expresses cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Insert

   cysteine-free GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30a-SARS-CoV2-NP

   Plasmid

   #165090

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pJH3449

   Plasmid

   #179576

   Purpose

   Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   BFP

   Expression

   Worm

   Mutation

   Promoter

   Plgc-55B

   Available since

   March 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. sgCTNNB1 Nicking

    Plasmid

    #169845

    Purpose

    Express a nicking sgRNA used for installation of the oncogenic S45F or S45del in Ctnnb1 in mouse liver.

    Depositor

    Wen Xue

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    sgCTNNB1 Nicking (Ctnnb1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid

    #170185

    Purpose

    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Inserts

    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PKT2/CLP-AKT

    Plasmid

    #20281

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    AKT (AKT1 Human)
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5 FLAG wildtype, catalytic dead lipin 1

    Plasmid

    #32006

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    Lipin1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D712E and D714E

    Promoter

    CMV, SP6

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry

    Plasmid

    #170184

    Purpose

    Expression vector for a sgRNA against BFP and spCas9-Sa163 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Sa163 msr-msd region by SpeI and AvrII.

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Inserts

    Sa163 RT
    Retron Sa163 msr-msd
    BFP-to-GFP conversion donor template An
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5 Flag Grb10 wt long

    Plasmid

    #37481

    Depositor

    David Sabatini

    Article

    Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

    Insert

    Grb10 (GRB10 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    isoform a, 594aa

    Promoter

    Available since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMKO.1 puro p53 shRNA 2

    Plasmid

    #10672

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-sACE2(WT)

    Plasmid

    #145147

    Purpose

    Mammalian expression plasmid for soluble ACE2 (protease domain)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

    Plasmid

    #102797

    Purpose

    Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Inserts

    sgRNA
    EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H231L

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEP4-myc-HLA-A1

    Plasmid

    #135503

    Purpose

    Mammalian expression of myc-tagged HLA-A*01:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*01:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET30a-Spike-E

    Plasmid

    #165096

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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