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pCHKU27.1-2.2

Plasmid

#111048

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_p.A772S

Plasmid

#82803

Purpose

Gateway Donor vector containing RB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RB1 (RB1 Human)

Use

Gateway Cloning

Mutation

A772S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_p.W140C

Plasmid

#82784

Purpose

Gateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway Cloning

Mutation

W140C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_p.A541G

Plasmid

#82771

Purpose

Gateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway Cloning

Mutation

A541G

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-SpyTag-PuroR

Plasmid

#80966

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

SpyTag

Use

Gene tagging

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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TAL2286

Plasmid

#36721

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HOXC13-TALEN_Left (HOXC13 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2287

Plasmid

#36722

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HOXC13-TALEN_Right (HOXC13 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pMBE1

Plasmid

#260675

Purpose

binary cloning vector

Depositor

John Vederas

Publication

van Belkum et al J Microbiol Methods. 2026 Sep 23;250:107

Type

Empty backbone

Use

Fungal expression

Promoter

gpdA promoter

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pBHM2616

Plasmid

#234495

Purpose

HYGR-CnU6-empty sgRNA

Depositor

Hiten Madhani

Publication

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

HYG-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:GST-AvrPWT4

Plasmid

#237522

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Publication

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

AvrPwt4

Expression

Bacterial

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-RWT4Ca

Plasmid

#237519

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Publication

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Expression

Bacterial

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:GST-VirPWT4

Plasmid

#237523

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Publication

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

VirPwt4

Expression

Bacterial

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR134.3_miRFP670

Plasmid

#163754

Purpose

gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Publication

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR134.3

Use

CRISPR

Tags

miRFP670

Expression

Mammalian

Promoter

U6

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHYX143

Plasmid

#202816

Purpose

Control plasmid expressing mScarlet-I fluorescent protein.

Depositor

Jean-Félix Dallery

Publication

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Insert

mScarlet-I

Expression

Yeast

Promoter

ScPCK1

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSC5GGv1_ShBle

Plasmid

#188603

Purpose

pSC5GGv1 with Sh ble insert for yeast selection

Depositor

Bogumil Karas

Publication

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-W160L+W275L

Plasmid

#196021

Purpose

Expresses CRKL W160L + W275L

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-W160L+Y207F

Plasmid

#196020

Purpose

Expresses Expresses CRKL W160L + Y207F

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCHKU42.1-2.2

Plasmid

#110672

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Tags

flag

Expression

Yeast

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU39.1-2.2

Plasmid

#110671

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Tags

flag

Expression

Yeast

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHKU36.1-2.2

Plasmid

#110668

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Tags

flag

Expression

Yeast

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge