We narrowed to 8,848 results for: sas

1. pCS2+-wbp2nlY55F

   Plasmid

   #172466

   Purpose

   expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nlY55F
   

   Use

   Xenopus expression

   Tags

   none

   Expression

   Mutation

   changed Tyrosine at aa55 to Phenylalanine

   Promoter

   SP6

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2+-wbp2nl

   Plasmid

   #172463

   Purpose

   expresses wild-type protein when injected into embryos

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nl (wbp2nl.L Frog)
   

   Use

   Xenopus expression

   Tags

   none

   Expression

   Mutation

   none

   Promoter

   SP6

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBAD-DiB1

   Plasmid

   #168800

   Purpose

   Fluorogen activating protein (FAP) DiB1

   Depositor

   Alexander Mishin

   Article

   Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

   Insert

   Fluorogen activating protein DiB1
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBAD-DiB3

   Plasmid

   #168802

   Purpose

   Fluorogen activating protein (FAP) DiB3

   Depositor

   Alexander Mishin

   Article

   Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

   Insert

   Fluorogen activating protein DiB3
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNAM-B1

   Plasmid

   #154056

   Purpose

   Level 0 Golden Gate vector, contains domesticated NAM-B1

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   TaNAM-B1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Synonymous mutation at base 3257, in exon 3, to r…

   Promoter

   N/A

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LLhE_AW-pHG165 H118R

   Plasmid

   #90063

   Purpose

   Constitutive expression of a chimeric LacI:CelR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Sousa et al J Mol Biol. 2016 Feb 22;428(4):671-8. do

   Insert

   LLhE_AW/H118R
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I48V/Q55A/Q60R/H118R

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCFJ150-lin26

   Plasmid

   #80357

   Purpose

   Pmex-5:GFP::H2B::PEST::lin-26 3'UTR reporter construct used for microinjection

   Depositor

   Sean Ryder

   Article

   Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

   Insert

   Pmex-5:GFP::H2B::PEST::lin-26 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCFJ150-atg4.2

   Plasmid

   #80352

   Purpose

   Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR reporter construct used for microinjection

   Depositor

   Sean Ryder

   Article

   Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

   Insert

   Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCFJ150-set6

   Plasmid

   #80362

   Purpose

   Pmex-5:GFP::H2B::PEST::set-6 3'UTR reporter construct used for microinjection

   Depositor

   Sean Ryder

   Article

   Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

   Insert

   Pmex-5:GFP::H2B::PEST::set-6 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCFJ150-ets4

    Plasmid

    #80355

    Purpose

    Pmex-5:GFP::H2B::PEST::ets-4 3'UTR reporter construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::ets-4 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCFJ150-mex3

    Plasmid

    #80361

    Purpose

    Pmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::mex-3 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCFJ150-mbk2

    Plasmid

    #80360

    Purpose

    Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO1-puro-U6-sgRNA-TS2

    Plasmid

    #69234

    Purpose

    U6 driven SpCas9 sgRNA expression for TS2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS2 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO1-puro-U6-sgRNA-TS3

    Plasmid

    #69235

    Purpose

    U6 driven SpCas9 sgRNA expression for TS3 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO1-puro-U6-sgRNA-TS4

    Plasmid

    #69236

    Purpose

    U6 driven SpCas9 sgRNA expression for TS4 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS4 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

    Plasmid

    #69221

    Purpose

    Expresses R1333K mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1333 is changed to Lysine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

    Plasmid

    #69222

    Purpose

    Expresses R1333S mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1333 is changed to Serine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2-F1-GGC

    Plasmid

    #30702

    Depositor

    Scot Wolfe, Nathan Lawson

    Article

    Zhu et al Development. 2011 Oct;138(20):4555-64.

    Insert

    F1 GGC
    

    Use

    Mrna production

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pBS-F1-GGG

    Plasmid

    #30703

    Depositor

    Scot Wolfe, Nathan Lawson

    Article

    Zhu et al Development. 2011 Oct;138(20):4555-64.

    Insert

    F1 GGG
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pBS-F1-GTA

    Plasmid

    #30705

    Depositor

    Scot Wolfe, Nathan Lawson

    Article

    Zhu et al Development. 2011 Oct;138(20):4555-64.

    Insert

    F1 GTA
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only