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Plasmid#194226PurposeLentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction markerDepositorInsertPGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTT3-ecdCD80-ST3-His
Plasmid#210665PurposeExpression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar)DepositorInsertExtracellular domain of human CD80 fused to Spytag003 and Histag (CD80 Human)
UseTagsHistag and Spytag003ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
KN501: pMVP (L3-L2) ecDHFR DD + WPRE
Plasmid#121793PurposepMVP L3-L2 entry plasmid, contains ecDHFR DD + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stablized by TMP) to gene in lentivirus vectorsDepositorInsertecDHFR Degron Domain + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-hTie1-ECD-FLAG
Plasmid#119235PurposePlasmid encodes FLAG-tagged extracellular domain of human Tie1 gene cloned in lentiviral vector.DepositorInsertTIE1-ECD-FLAG (TIE1 Human)
UseLentiviralTagsFLAGExpressionMutationContains extracellular domain (ECD) onlyPromoterP hUbCAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys-T46C
Plasmid#110543PurposeBacterial expression of E.coli DHFR C85A/C152S/T46C mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationT46 mutated to C46, C85 mutated to A85, C152 muta…PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys-L54C
Plasmid#110542PurposeBacterial expression of E.coli DHFR C85A/C152S/L54C mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationL54 mutated to C54, C85 mutated to A85, C152 muta…PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-D27S/Y100F
Plasmid#110827PurposeBacterial expression of E.coli DHFR D27S/Y100F mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationD27 mutated to S27, Y100 mutated to F100PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 ECD-MHC
Plasmid#113957Purposemammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class IDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG, Signal/leader sequence from influenza hemag…ExpressionMammalianMutationresidues 22-568 fused to a transmembrane tetherPromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HLA-c-myc-optiT1R3 ECD
Plasmid#113960Purposemammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R3 (TAS1R3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationresidues 21-563PromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)
Plasmid#167313PurposeTemplate pDNA to prepare DD-CaVT mRNADepositorInsertDD-CaVT
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only