Addgene

We narrowed to 259 results for: H2A

Showing: 161 - 180 of 259 results

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pCAG-RNF169UBD

Plasmid

#119006

Purpose

Mammalian expression of the human RNF169 Ubiquitin binding domain and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

RNF169 (RNF169 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pU6Rosa-CAG-Gal4-Rad18UBD

Plasmid

#119012

Purpose

Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Gal4 (GAL4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Gal4-RNF169UBD

Plasmid

#119013

Purpose

Mammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Gal4 (GAL4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-BRCA1

Plasmid

#119007

Purpose

Mammalian expression of human BRCA1 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

BRCA1 (BRCA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TLR6

Plasmid

#118997

Purpose

Targeting vector for human AAVS1 with TLR6 reporter insert

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

AAVS1 (PPP1R12C, AAVS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pETNKI-his-SUMO-RNF168ring (1-189)

Plasmid

#63570

Purpose

Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

RNF168 (RNF168 Human)

Use

Tags

6xHis and SUMO3

Expression

Bacterial

Mutation

Ring domain only (residues 1-189)

Promoter

T7

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-BRCA1-RNF169UBD

Plasmid

#119009

Purpose

Mammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

BRCA1 (BRCA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-BRCA1-Rad18UBD

Plasmid

#119008

Purpose

Mammalian expression of a fusion protein of human BRCA1 with human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

BRCA1 (BRCA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT/TO-eGFP-53BP1

Plasmid

#60813

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mRFP-Ub

Plasmid

#11935

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin (RBX1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-Ub KO

Plasmid

#11934

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin KO (RBX1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

All 7 lysines of ubiquitin mutated to arginines.

Promoter

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuc-GsCT

Plasmid

#205517

Purpose

Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

nuc-GsCT (GNAS Synthetic, Human)

Use

Tags

Histone H2A and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR-53BP1 1-1972 WT

Plasmid

#53446

Purpose

Recombinational donor/master vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Gateway

Tags

Expression

Mutation

siRNA resistant and mutations A128V, T720A, A1347…

Promoter

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

Plasmid

#60814

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Tags

eGFP-NLS (SV40)

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pIND-EGFP

Plasmid

#15770

Depositor

Danny Rangasamy

Insert

mH2A.Z-EGFP (H2az1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pDONR-53BP1 1-1972 28A

Plasmid

#53447

Purpose

Recombinational donor/master vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Gateway

Tags

Expression

Mutation

I914T mutation; All 28 SQ/TQ motifs in N-term of …

Promoter

Available since

Dec. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSMH1741

Plasmid

#160930

Purpose

Expression of soluble Sc octamer (H3)

Depositor

Stephen Harrison

Inserts

HHF1
HTA1
HTB1
HHT1

Use

Tags

6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…

Expression

Mutation

codon-optimized for E. coli -- see provided seque…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSMH1742

Plasmid

#160929

Purpose

Expression of soluble Sc octamer (Cse4)

Depositor

Stephen Harrison

Inserts

HHF1
HTA1
HTB1
Cse4

Use

Tags

6His-TEV, N-terminal 6His-TEV, and none

Expression

Mutation

codon-optimized for E. coli -- see provided seque…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGI3EM22C

Plasmid

#135488

Purpose

Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr LifeAct-tdTomato

Use

Tags

LifeAct

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM20C

Plasmid

#135487

Purpose

Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr H2B-tdTomato

Use

Tags

tdTomato

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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