We narrowed to 4,096 results for: SP6

1. pSP64T-ythdf3-3xflag

   Plasmid

   #164490

   Purpose

   Expresses zebrafish ythdf3 with 3x flag tag, for mRNA injection

   Depositor

   Antonio Giraldez

   Article

   Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

   Insert

   ythdf3 (ythdf3 Zebrafish)
   

   Use

   Production of mrna for zebrafish injection.

   Tags

   3x-flagtag

   Expression

   Mutation

   Promoter

   SP6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSP64-hERG1a D540K

   Plasmid

   #53057

   Purpose

   Expresses alpha subunit of D540K hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al J Physiol. 1999 Feb 1;514 ( Pt 3):667-75

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Aspartate 540 to Lysine

   Promoter

   SP6

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSP64-hERG1a S620T

   Plasmid

   #53059

   Purpose

   Expresses alpha subunit of S620T hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Casis et al Mol Pharmacol. 2006 Feb;69(2):658-65. Ep

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Serine 620 to Threonine

   Promoter

   SP6

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSP64-hERG1a S631A

   Plasmid

   #53058

   Purpose

   Expresses alpha subunit of S631A hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Zou et al J Physiol. 1998 May 15;509 ( Pt 1):129-3

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Serine 631 to Alanine

   Promoter

   SP6

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. HOXB4-FLAG-pSP65

   Plasmid

   #20990

   Depositor

   Corey Largman

   Article

   Chang et al Genes Dev. 1995 Mar 15. 9(6):663-74.

   Insert

   HOXB4 (HOXB4 Human)
   

   Use

   In vitro trans/trans

   Tags

   Expression

   Mutation

   Missing first 14 amino acids

   Promoter

   Available since

   Nov. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

6. HOXB6-FLAGA-pSP65

   Plasmid

   #20994

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

   Insert

   HOXB6 (HOXB6 Human)
   

   Use

   In vitro trans/trans

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pSP64T XAR1 (DM#105)

   Plasmid

   #15044

   Depositor

   Douglas Melton

   Article

   Hemmati-Brivanlou et al Nature. 1992 Oct 15. 359(6396):609-14.

   Insert

   Xenopus activin receptor 1 (xstk2 Frog)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pSP64TNE Xnr4 (DM#174)

   Plasmid

   #15078

   Depositor

   Douglas Melton

   Article

   Joseph et al Dev Biol. 1997 Apr 15. 184(2):367-72.

   Insert

   Xnr4 (xnr4 Frog)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pBR9-genomic_RSP6-HAX3

   Plasmid

   #176703

   Purpose

   Rescue of RSP6 Chlamydomonas mutant with tagged RSP6

   Depositor

   Ron Vale

   Article

   Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

   Insert

   RSP6
   

   Use

   Tags

   HAX3

   Expression

   Plant

   Mutation

   None

   Promoter

   rbcs2

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hsp68-LacZ-Gibson

    Plasmid

    #170102

    Purpose

    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.

    Depositor

    Len Pennacchio

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Mouse Hsp68 minimal promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SP6-VEE-GFP

    Plasmid

    #58976

    Purpose

    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    EGFP
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. SP6-VEE-OKS-iM

    Plasmid

    #58973

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SP6-VEE-OKS-iG

    Plasmid

    #58975

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Hsp68-LacZ-Gateway

    Plasmid

    #37843

    Depositor

    Nadav Ahituv

    Article

    Pennacchio et al Nature. 2006 Nov 23;444(7118):499-502. E

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Mouse Hsp68 minimal promoter

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSP6-EBNA(2A+DBD)

    Plasmid

    #98749

    Purpose

    In vitro transcription of modified EBNA1 mRNA

    Depositor

    Jim Vadolas, Sara Howden

    Article

    Howden et al Hum Gene Ther. 2006 Aug;17(8):833-44.

    Insert

    Modified EBNA1
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET23b-Casp6-His

    Plasmid

    #11823

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 6 (CASP6 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. Hsp68-CreER(TM) (DM#264)

    Plasmid

    #15028

    Depositor

    Douglas Melton

    Insert

    Cre-ER(TM)
    

    Use

    Cre/Lox; For making transgenic constructs

    Tags

    Hsp68 promoter and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEntry_HCoV-OC43-nsp6

    Plasmid

    #168934

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-OC43-nsp6 (ORF1ab )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSP64-hERG1a T623A

    Plasmid

    #53055

    Purpose

    Expresses alpha subunit of T623A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    changed Threonine 623 to Alanine

    Promoter

    SP6

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSP64-hERG1a S624A

    Plasmid

    #53056

    Purpose

    Expresses alpha subunit of S624A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    changed Serine 624 to Alanine

    Promoter

    SP6

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge