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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 413 results for: bcl 2

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Showing: 161 - 180 of 413 results

  1. Venus-Puma-4E-pEGFP-C1

    Plasmid
    #166746
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi
    Insert
    Puma-4E (BBC3 Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166746-plasmid-map-sequence-id-329007

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  8. Venus-Noxa-4E-pEGFP-C1

    Plasmid
    #166745
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Noxa with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi
    Insert
    Noxa-4E (PMAIP1 Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    April 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    166745-plasmid-map-sequence-id-330212

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  9. mCerulean3-Bik-s2193

    Plasmid
    #177425
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bik, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    Human ferritin
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177425-plasmid-map-sequence-id-347947

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  10. Venus-Bik-AA-pEGFP-C1

    Plasmid
    #177430
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with 2 sites of phosphorylation mutated to alanine. T33A and S35A mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with alanine
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177430-plasmid-map-sequence-id-348458

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  13. Venus-Bik-L61G-pEGFP-C1

    Plasmid
    #177426
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Leucine 61 replaced with glycine
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177426-plasmid-map-sequence-id-348449

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  14. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid
    #177427
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycines 153 and 154 mutated to alanine
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177427-plasmid-map-sequence-id-348452

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  15. Venus-Bik-G154STOP-pEGFP-C1

    Plasmid
    #177428
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycine 154 replaced with STOP codon
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177428-plasmid-map-sequence-id-348454

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  17. Venus-Bik-DD-pEGFP-C1

    Plasmid
    #177429
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with aspartic…
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177429-plasmid-map-sequence-id-348456

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  18. Venus-Bik-cb5-pEGFP-C1

    Plasmid
    #177431
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136) and replaced…
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177431-plasmid-map-sequence-id-348459

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  20. Venus-tBid-4E-pEGFP-C1

    Plasmid
    #166743
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi
    Insert
    tBid-4E (Bid Mouse)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166743-plasmid-map-sequence-id-329004

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Showing: 161 - 180 of 413 results