We narrowed to 4,686 results for: pcr

1. pCR8 JARID2

   Plasmid

   #114443

   Purpose

   Entry vector for gateway cloning containing human JARID2 coding sequence

   Depositor

   Adrian Bracken

   Article

   Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

   Insert

   JARID2 (JARID2 Human)
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCR3.1-mutLuc

   Plasmid

   #106458

   Purpose

   luciferase reporter, CMV promoter (3 NFkB sites mutated)

   Depositor

   Georg Weber

   Article

   He et al Biochem Biophys Res Commun. 2004 Aug 13;

   Insert

   luciferase reporter, CMV promoter (3 NFkB sites mutated)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   3 NFkB sites mutated

   Promoter

   Available since

   July 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPRa_all-in-one

   Plasmid

   #183695

   Purpose

   Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRISPomyces-FnCpf1

   Plasmid

   #129554

   Purpose

   Contains codon-optimized FnCpf1 and gRNA for streptomyces

   Depositor

   Mingzi M. Zhang, Fong Tian Wong

   Article

   Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

   Insert

   FnCpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcrDNA3.1-PRKACA

   Plasmid

   #100890

   Purpose

   Mammalian expression of PRKACA

   Depositor

   Sanford Simon

   Article

   Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

   Insert

   Protein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Isoform 1 wild type

   Promoter

   CMV

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-11b_PcrA_M6_H93A

   Plasmid

   #194156

   Purpose

   Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

   Depositor

   Taekjip Ha

   Article

   Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

   Insert

   Engineered helicase PcrA_M6
   

   Use

   Tags

   6x His tag and FLAG tag

   Expression

   Bacterial

   Mutation

   H93A, C96A, N187C, C247A, L384V, L409C

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCRISPRx-Sth1Cas9-L5

   Plasmid

   #140993

   Purpose

   Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

   Depositor

   Wilbert Bitter

   Article

   Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

   Inserts

   Sth1Cas9
   TetR
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified; Integrative in mycobactreia

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPR-cBEST

   Plasmid

   #125689

   Purpose

   C to T base editor for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

   Insert

   streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   ermE*/tipA

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCR4-24XPP7SL

   Plasmid

   #31864

   Depositor

   Robert Singer

   Article

   Larson et al Science. 2011 Apr 22;332(6028):475-8.

   Insert

   24xPP7 Stem Loop Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCruzHA SIRT1

    Plasmid

    #10962

    Depositor

    Toren Finkel

    Article

    Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPR-dCas9

    Plasmid

    #125687

    Purpose

    Gene knockdown for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spdCas9, sgRNA casette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCR8/GW:U6

    Plasmid

    #85981

    Purpose

    Golden Gate Level 0 cassette encoding the U6 promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    T. pseudonana U6 promoter
    

    Use

    CRISPR; Diatom (t. pseudonana)

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCR4 hNFATc1-EE

    Plasmid

    #17857

    Depositor

    Jerry Crabtree

    Article

    Northrop et al Nature. 1994 Jun 9. 369(6480):497-502.

    Insert

    NFATc1 (NFATC1 Human)
    

    Use

    Tags

    EE

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pCRISPR-mcBEST

    Plasmid

    #209416

    Purpose

    Plasmid for multiplexed cytosine base editing in streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

    Inserts

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    base editing cassette: PtipA ; sgRNAs: PkasO* an…

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPomyces-AsCas12j-2

    Plasmid

    #191655

    Purpose

    All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

    Depositor

    Fong Tian Wong

    Article

    Tan et al bioRxiv 2021.10.28.465406

    Insert

    AsCas12j-2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCR4-myco

    Plasmid

    #36872

    Depositor

    Mario Chin

    Insert

    myco
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCR-XL-rAkap9

    Plasmid

    #196867

    Purpose

    Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akap9 (Akap9 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac promoter and T7 promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPEY-Z3-Nat

    Plasmid

    #232100

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCralbp-DsRed

    Plasmid

    #11158

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    Cralbp promoter (Rlbp1 Mouse)
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPR::rpsL

    Plasmid

    #44505

    Purpose

    A crRNA expression plasmid specific to the rpsL allele.

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    CRISPR::rpsL (rpsL )
    

    Use

    CRISPR; E.coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge