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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 173 results for: pef gfp

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Showing: 161 - 173 of 173 results

  3. LLP780_SpyCatcher-KRAB-CO

    Plasmid
    #211773
    Purpose
    SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpyCatcher-KRAB
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect KRAB e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211773-plasmid-map-sequence-id-417365

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  4. LLP784_SnoopCatcher-DNMT3A-CO

    Plasmid
    #211777
    Purpose
    SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spy-EZH2
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect EZH2 e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211777-plasmid-map-sequence-id-417364

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  6. pPL001

    Plasmid
    #184750
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.
    Depositor
    Jef Boeke
    Publication
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    184750-plasmid-map-sequence-id-365374

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  7. pLenti-NPY-mNeonGreen

    Plasmid
    #232456
    Purpose
    Expresses human NPY-mNeonGreen fusion in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    NPY-mNeonGreen (NPY Human)
    Use
    Lentiviral
    Tags
    mNeonGreen
    Promoter
    EF1 alpha
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232456-plasmid-map-sequence-id-461197

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  11. LLP789_αGCN4-DNMT3A-CO

    Plasmid
    #211783
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    aGCN4-DNMT3A
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon optimised (CO) to detect DNMT3A…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    211783-plasmid-map-sequence-id-417155

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  13. pSRG12 (eft-3p::24xGCN4::t2a::tagbfp::h2b::tbb-2 3’UTR)

    Plasmid
    #245120
    Purpose
    SunTag reporter for live imaging of translation (when combined with scFv::GFP expression) in C. elegans
    Depositor
    Suzan Ruijtenberg
    Publication
    van der Salm et al Development. 2025 May 15;152(10):dev2044
    Inserts
    eft-3p
    24xGCN4
    T2A
    TagBFP (GLO)
    H2B
    tbb-2 3'UTR
    right recombination arm MosSCI cxTi10816
    Left recombination arm MosSCI cxTi10816
    Expression
    Bacterial and Worm
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    245120-plasmid-map-sequence-id-496430

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Showing: 161 - 173 of 173 results