We narrowed to 2,799 results for: fas

1. pHCas9-Nours

   Plasmid

   #107733

   Purpose

   Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Human optimized

   Promoter

   TEF1

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ414-PCY1

   Plasmid

   #98150

   Purpose

   Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSR-siKlf5

   Plasmid

   #41740

   Depositor

   Jonathan Katz

   Article

   Yang et al FASEB J. 2007 Feb;21(2):543-50. Epub 200

   Insert

   Klf5 (Klf5 Mouse)
   

   Use

   RNAi

   Available Since

   Jan. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. cycLuc_SbMVs

   Plasmid

   #119209

   Purpose

   Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   cyclic luciferase with SbMVp cleavage site
   

   Use

   Luciferase

   Tags

   AU1 tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNB813

   Plasmid

   #60161

   Purpose

   Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   yEVenus-PEST
   

   Tags

   PEST degron

   Expression

   Yeast

   Promoter

   SIC1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDx_mScarlet-I-SYFP2

   Plasmid

   #189761

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-I-SYFP2
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNB784

   Plasmid

   #60163

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   SIC1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-hSyntenin2b

   Plasmid

   #89437

   Purpose

   mammalian expression of EGFP tagged human Syntenin2b

   Depositor

   Fritz Rathjen

   Article

   Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

   Insert

   SDCBP2 (SDCBP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   *see below

   Promoter

   CMV

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGREAT17

   Plasmid

   #170905

   Purpose

   GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   p35SoTMV

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-syn-fRGECO1

    Plasmid

    #125243

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO1
    

    Expression

    Mammalian

    Mutation

    D323A

    Promoter

    synapsin

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET30b GluBP600n

    Plasmid

    #119835

    Purpose

    Production of recombinant GluBP600n high affinity protein in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    GluBP600n
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET41a iGlul

    Plasmid

    #119832

    Purpose

    Production of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGlul
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    T92A

    Promoter

    T7

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSM14

    Plasmid

    #101645

    Purpose

    Encodes the fluorescent protein efasCFP for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    efasCFP
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGB1a2R+pNOS_Red-F_NOSt

    Plasmid

    #170888

    Purpose

    GoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Reverse orientation

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    Red-F
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    p.S286Y Red mutant

    Promoter

    pNOS

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNB655

    Plasmid

    #60142

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Expression

    Yeast

    Mutation

    A4V

    Promoter

    MET17pr

    Available Since

    Feb. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGREAT4

    Plasmid

    #170892

    Purpose

    GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pG10-90

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZA27

    Plasmid

    #158505

    Purpose

    In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-4xMyc. Km plant selection marker.

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1-4xMyc (ZAR1 Mustard Weed)
    

    Tags

    4xMyc

    Expression

    Plant

    Mutation

    L17E

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNB695

    Plasmid

    #60144

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Promoter

    MET17pr

    Available Since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNB697

    Plasmid

    #60146

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    RdLuc
    

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Promoter

    MET17pr

    Available Since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. TAL3439

    Plasmid

    #47158

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-fastkd3-right (im:7147223 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only