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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,632 results for: ef1a

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Showing: 1801 - 1820 of 7632 results

  2. PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP

    Plasmid
    #117319
    Purpose
    Overexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron
    Depositor
    Volker Busskamp
    Article
    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(
    Insert
    hsa-miR-CTRL
    Use
    Transposon-mediated integration
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    miR-124 deleted
    Promoter
    Tet-inducible promoter
    Available Since
    Jan. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    117319-plasmid-map-sequence-id-231738

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  4. pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

    Plasmid
    #220613
    Purpose
    Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220613-plasmid-map-sequence-id-441115

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  5. pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid
    #220614
    Purpose
    Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    Use
    AAV and Cre/Lox
    Tags
    3x HA; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220614-plasmid-map-sequence-id-441114

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  7. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118158-plasmid-map-sequence-id-240404

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  8. LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA

    Plasmid
    #47458
    Purpose
    LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE (Neurog2)-GS-CIB1(NLS*,∆318-334)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available Since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47458-plasmid-map-sequence-id-72985

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  11. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118157
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118157-plasmid-map-sequence-id-240364

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  12. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121814
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121814-plasmid-map-sequence-id-237361

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  13. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Available Since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

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  15. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121819
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121819-plasmid-map-sequence-id-237368

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Showing: 1801 - 1820 of 7632 results