We narrowed to 8,664 results for: ef1a

1. pU6-sgCtrl_EF1a-Puro-T2A-BFP

   Plasmid

   #195500

   Purpose

   Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP, Puro, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

   Plasmid

   #185061

   Purpose

   Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445

   Depositor

   Bobby Koeleman

   Insert

   U6:pegRNA:scaffold:PBS+RT template
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-EF1a-ArgSen (-)P2A (-)NLS

   Plasmid

   #184690

   Purpose

   Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   ArgSen (-)P2A (-)NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1a-ArgSen (-)P2A (-)NLS

   Plasmid

   #184699

   Purpose

   Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   ArgSen (-)P2A (-)NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-fDIO-tdTomato-WPRE

   Plasmid

   #187112

   Purpose

   FLP-dependent expression of tdTomato under EF1a promoter

   Depositor

   Yongling Zhu

   Article

   Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Hygro-EF1A-RG-Linker-eGFP

   Plasmid

   #176071

   Purpose

   EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   RG
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Hygro-EF1A-KR-Linker-eGFP

   Plasmid

   #176066

   Purpose

   EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   KR
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV_EF1a_RalA(G23V,full)-P2A-Hygro_Barcode

   Plasmid

   #170249

   Purpose

   Barcoded lentiviral vector to express RalA(G23V, full) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   RALA (RALA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G23V

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

   Plasmid

   #170236

   Purpose

   Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CHUK (CHUK Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   S176E, S180E

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-KIF5AR280H-HA-IRES-Puro

    Plasmid

    #166953

    Purpose

    Lentiviral plasmid expressing HA-tagged KIF5A R280H protein with IRES-Puro from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A-R280H (Kif5a Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    KIF5A-R280H

    Promoter

    eF1a

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJM466 EF1a VP16-ZF43 (TUPV2)

    Plasmid

    #138730

    Purpose

    mMoClo TUPV, with EF1alpha promoter and VP16-ZFa in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF43
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a-3NLS-Klf4 TALE-GCN4

    Plasmid

    #120546

    Purpose

    Express 3xNLS-Klf4 TALE-GCN4 engineered to bind a site in the human KLF4 gene

    Depositor

    Yu Wang

    Article

    Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

    Insert

    NLS, Klf4 TALE, GCN4 (KLF4 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

    Plasmid

    #113159

    Purpose

    An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)

    Plasmid

    #110159

    Purpose

    Lentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

    Insert

    TFAP4 (transcription factor AP-4) (TFAP4 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E135A/S139A

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRRLNeo-pEF1a-p53ashL344A-mKate2-splitmVenusN

    Plasmid

    #69584

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC

    Plasmid

    #69585

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRH-1328 LV EF1a-CD20 IRES-EGFP

    Plasmid

    #201918

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD20
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR hEF1A-BFP-P2A-rtTA-WPRE

    Plasmid

    #223288

    Purpose

    A lentiviral vector expressing BFP and the TetON transcription factor rtTA.

    Depositor

    Jared Toettcher

    Article

    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

    Insert

    BFP-P2A-rtTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid

    #102244

    Purpose

    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

    Depositor

    Jacob Corn

    Insert

    dCas9-HA-BFP-KRAB-NLS
    

    Use

    CRISPR and Lentiviral

    Tags

    HA-TagBFP-KRAB-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204189

    Purpose

    Doxycycline inducible lentiviral vector for human GATA6 overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    GATA6 (GATA6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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