We narrowed to 7,951 results for: try
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Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneExpressionBacterial and MammalianPromoterU6Available SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only
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pET21-pelB-GST-8
Plasmid#228990PurposeFor bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-8
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
MBD-IFP2.0C
Plasmid#215748PurposeExpresses MBD1 MBD domain fused to C-terminal part of IFP2.0 for 5mC detection in BiAD sensorsDepositorInsertIFP2.0C
ExpressionMammalianPromoterCMVAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0817
Plasmid#177039PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa driven by 35S promoterDepositorInserttruncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa
UseSynthetic BiologyAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0784
Plasmid#177069PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of P19 suppressor of gene silencing from Tomato Bushy Stunt Virus driven by nos promoterDepositorInsertP19 suppressor of gene silencing from Tomato Bushy Stunt Virus
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0104
Plasmid#177038PurposeMoClo Level 1, position 6, transcriptional unit for transient expression of P19 suppressor of gene silencing from Tomato Bushy Stunt Virus driven by 35S promoterDepositorInsertP19 suppressor of gene silencing from Tomato Bushy Stunt Virus
UseSynthetic BiologyAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBad-EBFP2
Plasmid#14891PurposeBacterial expression of EBFP2 blue fluorescent proteinDepositorHas ServiceCloning Grade DNAInsertEBFP2
TagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…Available SinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
ExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV-sp-HPV-TM(ICAM)
Plasmid#196510PurposeExpress HPV antigen epitope with ICAM transmembrane domainDepositorInserta HPV epitope with CD8a stalk linker and ICAM TM domain
UseLentiviralTags3xflag tagExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_3
Plasmid#78200PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,I76G,A197C)
ExpressionBacterialMutationA17C,I76G,A197C (please see depositor comment bel…PromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD42-BtuCEDFB
Plasmid#237541Purposeplasmid encodes BtuC, BtuD, BtuE, BtuF, BtuB for enhanced solubilization of cobalamin proteinsDepositorInsertsbtuB
btuF
btuCED
ExpressionBacterialPromoterpBADAvailable SinceSept. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSecTag-ATX
Plasmid#17839DepositorInsertAutotaxin (ENPP2 Human)
ExpressionMammalianAvailable SinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-GpNLuc
Plasmid#70185PurposeConstiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterPGKAvailable SinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pirespuro2-CTLA4-mEos2
Plasmid#98285Purposemammalian expression of CTLA4-mEos2DepositorAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule-tfmF A65V S158A
Plasmid#85484PurposeMachinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNADepositorInserttri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
TagsNoneExpressionBacterialMutationY32V F108W Q109M I159M with 65Val and 158AlaPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP 150TAG
Plasmid#85483PurposeArabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
ExpressionBacterialMutation150TAG (amino acid 150 mutated to stop codon)PromoterArabinoseAvailable SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mucin MUC1-5TR-pET15b
Plasmid#174694PurposePlasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)DepositorInsertMUC1-TR
Tagshexahistidine (HIS) tagExpressionBacterialMutationnonePromoterT7 PromoterAvailable SinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmApple
Plasmid#191259PurposeBacterial expression plasmids for Long Stokes shift red fluorescent protein LSSmAppleDepositorInsertLSSmApple
TagsHis tagExpressionBacterialAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4-mammalian-recoded_mCherry_pcDNA3.1
Plasmid#114595PurposeEncodes mCherry-tagged BcLOV4 for expression in mammalian cellsDepositorInsertBcLOV4
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMito-MtrxRFP2
Plasmid#172324Purposefluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)DepositorInsertmitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
TagsHis-tag and Mitochondria localization sequenceExpressionMammalianPromoterCMV, SP6Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only