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Showing: 921 - 930 of 11158 results

  1. pLKO_FLAG.V5.C_NGFR

    Plasmid
    #158259
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG.V5.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158259-plasmid-map-sequence-id-327882

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  2. pLKO_HA.AU1.NWS_NGFR

    Plasmid
    #158234
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.AU1.NWS
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158234-plasmid-map-sequence-id-334982

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  3. pLKO_HA.AU1.FLAG_NGFR

    Plasmid
    #158236
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.AU1.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158236-plasmid-map-sequence-id-334983

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  4. pLKO_NWS.AU1.V5_NGFR

    Plasmid
    #158237
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.AU1.V5
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158237-plasmid-map-sequence-id-327677

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  5. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid
    #232116
    Purpose
    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    BCKDK (BCKDK Human)
    Use
    Tags
    6 x His, Biotin acceptor peptide (BAP), Maltose-B…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232116-plasmid-map-sequence-id-464924

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  8. pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

    Plasmid
    #216871
    Purpose
    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23
    Depositor
    Ori Bar-Nur
    Article
    Lenardic et al J Clin Invest. 2024 May 7:e166998. doi:
    Insert
    Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    mdx mutation in mouse dystrophin
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    216871-plasmid-map-sequence-id-435357

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  9. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    DBT (DBT Human)
    Use
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232117-plasmid-map-sequence-id-465790

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  10. pKT-IDH1(R132H)-IRES-Katushka

    Plasmid
    #124257
    Purpose
    Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Article
    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii
    Insert
    Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Arginine 132 is mutated to Histidine. This mutati…
    Promoter
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124257-plasmid-map-sequence-id-252091

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Showing: 921 - 930 of 11158 results