We narrowed to 7,363 results for: ALP

1. pHAGE2-TetO-Chim-Salhel-I-K57E

   Plasmid

   #231519

   Purpose

   Expresses Salpingoeca helianthica chimeric Sox-I mutant in mammalian cells, for lentivirus generation

   Depositor

   Ralf Jauch

   Article

   Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

   Insert

   Salhel-I HMG K57E flanked with N- and C-termini of mSox2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Point mutation at position 57 K to E of the HMG

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE2-TetO-Salro-Sox-like

   Plasmid

   #231531

   Purpose

   Expresses Salpingoeca rosetta full-length Sox-like in mammalian cells, for lentivirus generation

   Depositor

   Ralf Jauch

   Article

   Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

   Insert

   Sox-like
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-6P-acatenin

   Plasmid

   #186458

   Purpose

   Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

   Depositor

   Tina Izard

   Article

   Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET41SIII-ATF6(1-373)

   Plasmid

   #171074

   Purpose

   Expresses of ATF6α(1-373) in bacteria

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   ATF6α (ATF6 Human)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cav2.2 HA pCAGGS

   Plasmid

   #206052

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/B-actin

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCellFree_G03 AKT1

   Plasmid

   #67102

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. Gal4pBS134-Pleum

   Plasmid

   #60339

   Purpose

   Contains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. PIK3CA - Exon9 E545K

   Plasmid

   #16642

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Science. 2004 Apr 23. 304(5670):554.

   Insert

   PIK3CA E545K (PIK3CA Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E545K; see note below

   Promoter

   Available since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. mouse E-cadherin GFP (423)

   Plasmid

   #67937

   Purpose

   Mammalian expression of E-cadherin-GFP

   Depositor

   Alpha Yap

   Article

   Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

   Insert

   E-cadherin (Cdh1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPW3758 : CMVd3-HsIRE1-HaloTag

    Plasmid

    #185680

    Purpose

    Low-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. FR_HNF4A2

    Plasmid

    #31100

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-IpgD

    Plasmid

    #183666

    Purpose

    N-terminally tagged Shigella flexneri IpgD for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pECE HA AMPKa1 WT

    Plasmid

    #69504

    Purpose

    Expresses AMPK alpha1 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. CaV1.3e[8a,11,31b,Δ32,42a]

    Plasmid

    #49333

    Purpose

    Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, 31b and 42a.Deletion of ex…

    Promoter

    CMV

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Human a4 nicotinic subunit crystallization construct

    Plasmid

    #85841

    Purpose

    pEZT-BM vector with alpha4 gene after CMV promoter, published crystallization construct

    Depositor

    Ryan Hibbs

    Article

    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

    Insert

    Human a4 nAChR subunit crystallization construct (CHRNA4 Human)
    

    Use

    Baculovirus (bacmam) production and mammalian exp…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

    Plasmid

    #185676

    Purpose

    SpCas9 and gRNA targeting the C-terminus of HsIRE1

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 gRNA (ERN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

17. Cav2.2 BBS pcDNA3

    Plasmid

    #206057

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II

    Depositor

    Annette Dolphin

    Article

    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. ACTN3-pEGFP

    Plasmid

    #52670

    Purpose

    Overexpression of GFP-tagged α-actinin-3

    Depositor

    Johannes Hell

    Article

    Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

    Insert

    ACTN3 (ACTN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pM2AH + mSlo1

    Plasmid

    #16152

    Depositor

    Larry Salkoff

    Article

    Butler et al Science. 1993 Jul 9. 261(5118):221-4.

    Insert

    Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-RIIα-Dronpa

    Plasmid

    #182331

    Purpose

    PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    RIIalpha-Dronpa (PRKAR2A Human)
    

    Use

    Tags

    Full-length human PKA type 2alpha regulatory subu…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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