We narrowed to 2,128 results for: D10

1. pGFP-mTet1s_K852R pc3914

   Plasmid

   #201704

   Purpose

   Expresses of GFP-tagged mTet1s_K852R in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1s-K852R (Tet1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lysine 852 mutated to arginine

   Promoter

   CAG

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGFP-mTet1s_K852E pc3915

   Plasmid

   #201705

   Purpose

   Expresses of GFP-tagged mTet1s_K852E in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1s-K852E (Tet1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lysine 852 mutated to glutamate

   Promoter

   CAG

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSCBE3-HF1

   Plasmid

   #218156

   Purpose

   This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-HF1
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N497A…

   Promoter

   rpsL promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSCBE3-NG

   Plasmid

   #218159

   Purpose

   This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-NG
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / L1111…

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSCBE3-HF1-Hypa

   Plasmid

   #218158

   Purpose

   This plasmid harbors the base editor SCBE3-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-HF1-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N497A…

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSCBE3-Hypa

   Plasmid

   #218157

   Purpose

   This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N692A…

   Promoter

   rpsL promoter

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSI-218

   Plasmid

   #197139

   Purpose

   All-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unit

   Depositor

   Nozomu Yachie

   Article

   Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   SpCas9-PmCDA1-UGI

   Expression

   Mammalian

   Mutation

   SpCas9(D10A)

   Promoter

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIG-713pUC19-tNGFR-P2A-CD45

   Plasmid

   #186109

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CD45 (PTPRC Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNOC_dCas9_sgRNA Td2

   Plasmid

   #176258

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   dead spCas9
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Expression

   Mutation

   H840A and D10A mutations on spCas9 to inactivate …

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. E1b-GFP-Tol2-Gateway dre SE-irf2bpl C

    Plasmid

    #89372

    Purpose

    Vector for reporter assay of one zebrafish super-enhancer region

    Depositor

    Alena Shkumatava

    Article

    Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

    Insert

    irf2bpl (irf2bpl Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. E1b-GFP-Tol2-Gateway dre SE-irf2bpl B

    Plasmid

    #89371

    Purpose

    Vector for reporter assay of one zebrafish super-enhancer region

    Depositor

    Alena Shkumatava

    Article

    Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

    Insert

    irf2bpl (irf2bpl Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. E1b-GFP-Tol2-Gateway dre SE-irf2bpl D

    Plasmid

    #89373

    Purpose

    Vector for reporter assay of one zebrafish super-enhancer region

    Depositor

    Alena Shkumatava

    Article

    Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

    Insert

    irf2bpl (irf2bpl Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. E1b-GFP-Tol2-Gateway dre SE-irf2bpl A

    Plasmid

    #89370

    Purpose

    Vector for reporter assay of one zebrafish super-enhancer region

    Depositor

    Alena Shkumatava

    Article

    Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

    Insert

    irf2bpl (irf2bpl Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid

    #129025

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

    Plasmid

    #129026

    Purpose

    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pD649-HAsp-SEMA7A-COMP5AP-AviTag-9xHis

    Plasmid

    #157486

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA7A (SEMA7A Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid

    #73497

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB-P2A-mCherry
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid

    #208336

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CAG

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. dCas9-CBP core

    Plasmid

    #179543

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge