Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,916 results for: FAST

Showing: 1841 - 1860 of 1916 results

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RAT +R111E

Plasmid

#187425

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Publication

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1 (Atp1a1 Rat)
ATP1B1 (Atp1b1 Rat)

Expression

Insect

Mutation

changed arginine at 118 to glutamic acid

Promoter

PH and p10

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFB HTB_hMSH6_T1219D

Plasmid

#135466

Purpose

Expression human MSH6_T1219D mutant protein using baculovirus

Depositor

Peggy Hsieh

Publication

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6_T1219D (MSH6 Human)

Tags

His-tag

Expression

Insect

Mutation

changed threonine to aspartic acid

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_EIF2A:1-427

Plasmid

#210939

Purpose

Insect expression of human EIF2A fragment M1 to T427. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF2A:M1-T427 (EIF2A Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_IFT172:1-320

Plasmid

#210934

Purpose

Insect expression of human IFT172 fragment M1 to S320. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

IFT172:M1-S320 (IFT172 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_TRAF7:382-670

Plasmid

#210930

Purpose

Insect expression of human TRAF7 fragment P382 to C670. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TRAF7:P382-C670 (TRAF7 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_CORO7:1-925

Plasmid

#210921

Purpose

Insect expression of full-length human CORO7. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO7:M1-D925 (CORO7 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_ATG16L2:321-619

Plasmid

#210918

Purpose

Insect expression of human ATG16L2 fragment A321 to Q619. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ATG16L2:A321-Q619 (ATG16L2 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_UTP4:1-686

Plasmid

#210915

Purpose

Insect expression of full-length human UTP4. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

UTP4:M1-R686 (CIRH1A Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_DCAF13:54-356

Plasmid

#210908

Purpose

Insect expression of human DCAF13 fragment F54 to A356. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF13:F54-A356 (DCAF13 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

A86S sequence conflict

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR12:90-417

Plasmid

#210903

Purpose

Insect expression of human WDR12 fragment T90 to T417. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR12:T90-T417 (WDR12 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

introduced mutations F99A K131A R319S

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_RACK1:1-317

Plasmid

#210890

Purpose

Insect expression of full-length human RACK1. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RACK1:M1-R317 (RACK1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_SMU1:170-513

Plasmid

#210882

Purpose

Insect expression of human SMU1 fragment K170 to P513. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SMU1:K170-P513 (SMU1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR91:392-747

Plasmid

#210878

Purpose

Insect expression of human WDR91 fragment P392 to A747. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:P392-A747 (WDR91 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR12:90-422

Plasmid

#210893

Purpose

Insect expression of human WDR12 fragment T90 to G422. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR12:T90-G422 (WDR12 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR41:30-459

Plasmid

#210892

Purpose

Insect expression of human WDR41 fragment Q30 to V459. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR41:Q30-V459 (WDR41 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR91:1-747

Plasmid

#210879

Purpose

Insect expression of full-length human WDR91. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:M1-A747 (WDR91 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Chinchilla+N122D–insM

Plasmid

#191227

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Publication

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

removed methionine at 122 and replaced asparagine…

Promoter

PH and p10

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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S+Q111R+N122D

Plasmid

#167175

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cells

Depositor

Susanne Dobler

Publication

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-S+Q111R+N122D
ATP1B1

Expression

Bacterial and Insect

Mutation

Changed Q at 111 to R and changed N at 122 to D

Promoter

P10 and PH

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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mWasabi-ADAP1

Plasmid

#163906

Purpose

Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

Depositor

Martin Loose

Publication

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

mWasabi fluorescent tag

Expression

Insect

Promoter

polyhedrin

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt13C(WT)

Plasmid

#86576

Purpose

Expresses cleavable human full-length Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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Showing: 1841 - 1860 of 1916 results

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