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pET-PLS-KP6-C93A-C96A_rpoA-C

Plasmid

#190970

Purpose

Test the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:C93A/C96A

Promoter

T7

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP6-A66S_rpoA-C

Plasmid

#190988

Purpose

Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Use

Tags

His

Expression

Mammalian

Mutation

DYW-KP:A66S

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP6-C93A-C96A_rpoA-U

Plasmid

#190991

Purpose

Test the U-to-C editing activity of KP6:C93A/C96A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Use

Tags

His

Expression

Mammalian

Mutation

DYW-KP:C93A/C96A

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP2-C93A-C96A_rpoA-U

Plasmid

#190958

Purpose

Test the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:C93A/C96A

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP2-E67A_rpoA-U

Plasmid

#190979

Purpose

Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Use

Tags

His

Expression

Mammalian

Mutation

DYW-KP:E67A

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP3-E67A_rpoA-U

Plasmid

#190983

Purpose

Test the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Use

Tags

His

Expression

Mammalian

Mutation

DYW-KP:E67A

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP3-C93A-C96A_rpoA-U

Plasmid

#190982

Purpose

Test the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Use

Tags

His

Expression

Mammalian

Mutation

DYW-KP:C93A/C96A

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP6-C93A-C96A_rpoA-U

Plasmid

#190971

Purpose

Test the U-to-C editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Use

Tags

His

Expression

Bacterial

Mutation

DYW-KP:C93A/C96A

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP6-C93A-C96A_rpoA-C

Plasmid

#190990

Purpose

Test the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Use

Tags

His

Expression

Mammalian

Mutation

DYW-KP:C93A/C96A

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP2-C93A-C96A_rpoA-U

Plasmid

#190978

Purpose

Test the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Use

Tags

His

Expression

Mammalian

Mutation

DYW-KP:C93A/C96A

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP3-A66S_rpoA-U

Plasmid

#190981

Purpose

Test the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Use

Tags

His

Expression

Mammalian

Mutation

DYW-KP:A66S

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP6-E67A_rpoA-U

Plasmid

#190993

Purpose

Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Use

Tags

His

Expression

Mammalian

Mutation

DYW-KP:E67A

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMM4_14

Plasmid

#183062

Purpose

Plasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

Inserts

mCherry
BM3R1-HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Mutation

Promoter

HREsYGP1p and RET2

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mVenus-FAK(397)FRB

Plasmid

#188660

Purpose

mVenus tagged Focal Adhesion Kinase with FRB inserted at Y397

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

Focal Adhesion Kinase

Use

Tags

mVenus

Expression

Mammalian

Mutation

Insertion of FRB at Tyrosine 397

Promoter

CMV

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJS697

Plasmid

#168778

Purpose

Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Type

Empty backbone

Use

Tags

Cmyc and HA

Expression

Yeast

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

Plasmid

#153443

Purpose

E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.XDocB

Use

Tags

6xHis and ybbr tag

Expression

Bacterial

Mutation

Serine 199 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFR093

Plasmid

#134149

Purpose

Expression of CENP-T

Depositor

Iain Cheeseman

Article

Nishino et al EMBO J. 2013 Feb 6;32(3):424-36. doi: 10

Insert

CENP-T (CENPT Human)

Use

Tags

His

Expression

Bacterial

Mutation

aa 1-375 with T85D mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAL832

Plasmid

#119305

Purpose

precusor outer membrane protein A, ompA, truncated after amino acid 176

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

precursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C

Use

Tags

none

Expression

Bacterial

Mutation

truncated after amino acid 176, mutations Threoni…

Promoter

lac

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAL800

Plasmid

#119306

Purpose

precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S

Use

Tags

none

Expression

Bacterial

Mutation

amino acids 1 through 171 deleted, and mutatioms …

Promoter

lac

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MAGE-112-429

Plasmid

#89128

Purpose

mammalian expression of human MAGE-A11 amino acid residues 112-429 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 112-429

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only