We narrowed to 4,699 results for: golden gate

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DENV-3 strain Phillippines/H87/1956 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228651

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 3 for biochemical assays

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue 3 portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag

Plasmid

#228635

Purpose

Bacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interaction

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus A71 3C protease isoform A

Tags

His-Avi

Expression

Bacterial

Available Since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag

Plasmid

#228634

Purpose

Bacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interaction

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus Coxsackievirus A71 2A protease

Tags

His-Avi

Expression

Bacterial

Available Since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Human Coxsackievirus A16 strain G10 2A protease

Plasmid

#228632

Purpose

Bacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA)

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus Coxsackievirus A16 2A protease (HEVAgp1 Coxsackievirus 16)

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pVP311

Plasmid

#222070

Purpose

MultiGreen 2.0 AB level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP312

Plasmid

#222071

Purpose

MultiGreen 2.0 BC level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP313

Plasmid

#222072

Purpose

MultiGreen 2.0 CD level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP314

Plasmid

#222073

Purpose

MultiGreen 2.0 DE level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP315

Plasmid

#222074

Purpose

MultiGreen 2.0 EF level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP316

Plasmid

#222075

Purpose

MultiGreen 2.0 FG level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP078

Plasmid

#222030

Purpose

MultiGreen 2.0 AB level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP079

Plasmid

#222031

Purpose

MultiGreen 2.0 BC level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP080

Plasmid

#222032

Purpose

MultiGreen 2.0 CD level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP081

Plasmid

#222033

Purpose

MultiGreen 2.0 DE level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP082

Plasmid

#222034

Purpose

MultiGreen 2.0 EF level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP083

Plasmid

#222035

Purpose

MultiGreen 2.0 FG level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGGPK-AG2

Plasmid

#222027

Purpose

Empty Kanamycin resistant GreenGate destination vector based on pGGP-AG.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBH111

Plasmid

#215778

Purpose

NBU2 backbone with anti-LacI(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

anti-LacI(YQR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(O1)

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH101

Plasmid

#215772

Purpose

NBU2 backbone with LacI(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(YQR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

BT1311 and P(O1)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH102

Plasmid

#215773

Purpose

NBU2 backbone with LacI(TAN) and P(tta)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(TAN)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(tta)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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