We narrowed to 3,437 results for: multiplex

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CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#194726

Purpose

CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector

Depositor

Rudolf Jaenisch

Publication

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1(D908A)

Use

CRISPR

Expression

Mammalian

Mutation

D908A

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-TRE3G-Nluc

Plasmid

#192659

Purpose

A plasmid encoding TRE3G sgRNA and TRE3G-promoter-Nluc

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Nluc

Use

Luciferase

Expression

Mammalian

Promoter

U6, TRE3G

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-Nluc

Plasmid

#192657

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Nluc

Use

Luciferase

Expression

Mammalian

Promoter

U6, miniCMV

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-INS-sg

Plasmid

#194719

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINS

Depositor

Rudolf Jaenisch

Publication

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (INS Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-MAFB-sg

Plasmid

#194718

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hMAFB

Depositor

Rudolf Jaenisch

Publication

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (MAFB Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-GFP-sg

Plasmid

#194717

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target GFP

Depositor

Rudolf Jaenisch

Publication

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1

Use

CRISPR

Expression

Mammalian

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL1RN gRNA_1-MS2-Puro

Plasmid

#192677

Purpose

Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNA #1 (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_STf

Plasmid

#182000

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines

Depositor

Kai Johnsson

Publication

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-TagRFP-LaminB1

Plasmid

#184448

Purpose

Nuclear memebrane-targeted TagRFP expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Publication

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

TagRFP-LaminB1

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-TagRFP-CAAX

Plasmid

#184447

Purpose

Plasma membrane-targeted TagRFP expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Publication

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

TagRFP-CAAX

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-NLS

Plasmid

#184450

Purpose

Nucleus-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Publication

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-3xNLS

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVITRO1-mCardinal-LaminB1

Plasmid

#184452

Purpose

Nuclear memebrane-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Publication

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-LaminB1

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVITRO1-BFP-NES

Plasmid

#184461

Purpose

Cytosolic EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Publication

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-NES

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMetLuc

Plasmid

#135286

Purpose

GBPart - MetLuc Luciferase CDS

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Metridia Luciferase

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-CT-Tagging

Plasmid

#165871

Purpose

Template for amplification of EGFP C-terminal tagging cassette

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - CT Tagging

Use

Synthetic Biology

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p3xP3

Plasmid

#165779

Purpose

3xP3 eye driver enhancer element, requires promoter element for function

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

3xP3

Expression

Insect

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry TL1

Plasmid

#165832

Purpose

mCherry red fluorescent protein for N-terminal tagging

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

mCherry

Expression

Insect

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165888

Purpose

Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-CT-Tagging

Plasmid

#165869

Purpose

Template for amplification of mcherry C-terminal tagging cassette

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - CT Tagging

Use

Synthetic Biology

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-NT-Tagging

Plasmid

#165868

Purpose

Template for amplification of EGFP N-terminal tagging cassette

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - NT Tagging

Use

Synthetic Biology

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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