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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,792 results for: plat

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Showing: 1841 - 1860 of 8792 results

  3. LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)

    Plasmid
    #211767
    Purpose
    dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9, Spy, Snoop, aGCN4, AviTags
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    dCas9 D10A and H840A
    Promoter
    pGK and pEF1a
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211767-plasmid-map-sequence-id-417383

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  4. pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid
    #229767
    Purpose
    Human Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system
    Depositor
    Hirofumi Kai
    Publication
    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    Use
    Luciferase
    Tags
    SmBiT
    Mutation
    See Depositor Comments
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229767-plasmid-map-sequence-id-457423

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  5. Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R]

    Plasmid
    #177486
    Purpose
    Mammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/60.
    Depositor
    James Trimmer
    Publication
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Slo1/BKAlpha maxi-K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnma1 Mouse)
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available since
    Nov. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    177486-plasmid-map-sequence-id-347922

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  6. AICSDP-55:DMD-mEGFP

    Plasmid
    #159744
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159744-plasmid-map-sequence-id-314374

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  8. pATP416-­pluxO5-­pphlO6-­crtYBI

    Plasmid
    #165977
    Purpose
    Expresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTA
    Depositor
    Jun Ishii
    Publication
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Expression
    Yeast
    Mutation
    C66A, C1359T and C1281A, G1698A
    Promoter
    Synthetic promoter (pluxO5), Synthetic promoter (…
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    165977-plasmid-map-sequence-id-328856

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  9. p28-2iDP-puro

    Plasmid
    #88893
    Purpose
    Dual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.
    Depositor
    Michele Calos
    Publication
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Dual-intron DMD platform
    luciferase
    puromycin resistance enzyme
    mCherry
    Expression
    Mammalian
    Mutation
    Truncated version of the dystrophin protein (tran…
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    88893-plasmid-map-sequence-id-201484

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  12. pRS263

    Plasmid
    #122379
    Purpose
    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon
    Depositor
    Rebecca Shapiro
    Publication
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Insert
    HSP90
    Use
    CRISPR
    Expression
    Yeast
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122379-plasmid-map-sequence-id-241246

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  13. pRS267

    Plasmid
    #122380
    Purpose
    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon
    Depositor
    Rebecca Shapiro
    Publication
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Insert
    HSP90
    Use
    CRISPR
    Expression
    Yeast
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122380-plasmid-map-sequence-id-243382

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  15. pAAH2

    Plasmid
    #41826
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Publication
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7, TEF
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41826-plasmid-map-sequence-id-69077

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  19. pIK198

    Plasmid
    #65629
    Purpose
    "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.
    Depositor
    Iskra Katic
    Publication
    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Worm
    Promoter
    U6
    Available since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65629-plasmid-map-sequence-id-111540

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Showing: 1841 - 1860 of 8792 results