We narrowed to 4,245 results for: Pet2

1. Sars Cov 2 MPRO R188S mutantSARS-CoV-2 Mpro protease (R188S mutant - clinical mutant); aliases: 3CLpro, 3C-like

   Plasmid

   #204785

   Purpose

   Bacterial expression of Sars Cov 2 MPRO R188S mutant

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R188S

   Promoter

   Available since

   Jan. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. SARS-CoV-2 Mpro protease (R188K mutant - clinical mutant); aliases: 3CLpro, 3C-like

   Plasmid

   #204770

   Purpose

   Bacterial expression of Sars Cov 2 MPRO R188K mutant

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R188K col1

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pDL28_Cas9-His Δcys E532C/E1207C

   Plasmid

   #206289

   Purpose

   Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S, E1207C

   Promoter

   T7

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDL25_Cas9-His Δcys E1207C

   Plasmid

   #206286

   Purpose

   Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1207C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDL23_Cas9-His Δcys E945C

   Plasmid

   #206284

   Purpose

   Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E945C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDL24_Cas9-His Δcys E1026C

   Plasmid

   #206285

   Purpose

   Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1026C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDL27_Cas9-His Δcys E532C/E945C

   Plasmid

   #206288

   Purpose

   Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S, E945C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDL26_Cas9-His Δcys M1C/E532C

   Plasmid

   #206287

   Purpose

   Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, E532C, C574S

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDL22_Cas9-His Δcys E532C

   Plasmid

   #206283

   Purpose

   Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBEL2572

    Plasmid

    #195683

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(V171R)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(V171R)

    Promoter

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBEL1223

    Plasmid

    #195688

    Purpose

    Expression/purification of 6xHis-TEV-MlaDdelta173-183

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaDdelta173-183

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEL1909

    Plasmid

    #195690

    Purpose

    Expression/purification of 6xHis-TEV-MlaD(F118A)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaD(F118A)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEL1910

    Plasmid

    #195691

    Purpose

    Expression/purification of 6xHis-TEV-MlaD(L123A)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaD(L123A)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEL2555

    Plasmid

    #195673

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(G170K)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(G170K)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBEL2556

    Plasmid

    #195674

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(I130D)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(I130D)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL2559

    Plasmid

    #195675

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(L76D)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(L76D)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEL2560

    Plasmid

    #195676

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(N155A)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(N155A)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEL2561

    Plasmid

    #195677

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(N179E)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(N179E)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBEL2563

    Plasmid

    #195678

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(R134E)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(R134E)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEL2564

    Plasmid

    #195679

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(R147D)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(R147D)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBEL2565

    Plasmid

    #195680

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(R153A)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(R153A)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBEL2566

    Plasmid

    #195681

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(R186A)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(R186A)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBEL2568

    Plasmid

    #195682

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(T176R)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(T176R)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBEL2573

    Plasmid

    #195684

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(Y72A)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(Y72A)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBEL2574

    Plasmid

    #195685

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(Y82K)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(Y82K)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBEL2576

    Plasmid

    #195686

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(Y164R)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(Y164R)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBEL1224

    Plasmid

    #195687

    Purpose

    Expression/purification of 6xHis-TEV-MlaDdelta153-183

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaDdelta153-183

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBEL2552

    Plasmid

    #195670

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(A168R)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(A168R)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBEL2553

    Plasmid

    #195671

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(D165I)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(D165I)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBEL2554

    Plasmid

    #195672

    Purpose

    Expression/purification of 6xHis-TEV-MlaC(E169R)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaC(E169R)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBEL1160

    Plasmid

    #196025

    Purpose

    Expression/purification of 6xHis-TEV-MlaD

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBEL1161

    Plasmid

    #196026

    Purpose

    Expression/purification of 6xHis-TEV-MlaDdelta141-183

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-MlaDdelta141-183

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-28a(+)-His(6)-TEV-AtPCO5(wt)

    Plasmid

    #191803

    Purpose

    Full length wt AtPCO5 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO5
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-28a(+)-His(6)-AtPCO1(wt)

    Plasmid

    #191798

    Purpose

    Full length wt AtPCO1 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO1
    

    Use

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET-28a(+)-His(6)-AtPCO2(wt)

    Plasmid

    #191799

    Purpose

    Full length wt AtPCO2 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO2
    

    Use

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-28a(+)-His(6)-AtPCO3(wt)

    Plasmid

    #191800

    Purpose

    Full length wt AtPCO3 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO3
    

    Use

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET-PLS-KP7_rpoA-U

    Plasmid

    #190974

    Purpose

    Test the U-to-C editing activity of KP7 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP7
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET-PLS-KP2-E67A_rpoA-U

    Plasmid

    #190959

    Purpose

    Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-PLS-KP6-A66S_rpoA-C

    Plasmid

    #190968

    Purpose

    Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET-PLS-KP1_rpoA-U

    Plasmid

    #190955

    Purpose

    Test the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge