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pJ211_rha_WT_low

Plasmid

#128851

Purpose

Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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alpha-actinin-mGarnet2

Plasmid

#104316

Purpose

codes for a fusion of fluorescent protein mGarnet2 and human alpha-actin, visualizes human alpha-actinin

Depositor

Ulrich Nienhaus

Article

Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

Insert

alpha-actinin-mGarnet2

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHis17_ParM _1

Plasmid

#78201

Purpose

Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

Depositor

Martin Webb

Article

Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

Insert

parM (K33A I27C T174A T175N C287A)

Tags

His6

Expression

Bacterial

Mutation

K33A I27C T174A T175N C287A

Promoter

unknown

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Orange_CaMBI_300

Plasmid

#124095

Purpose

orange calcium-modulated bioluminescent indicator, 300nM

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Orange CaMBI 300

Expression

Mammalian

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Green Falcan0.3

Plasmid

#199704

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan0.3

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJS96

Plasmid

#207588

Purpose

GFP-TonBH20A expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TonBH20A

Expression

Bacterial

Mutation

H20A

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Green Falcan3

Plasmid

#199706

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan3

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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prsTagRFP-C1

Plasmid

#31926

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mIFP

Plasmid

#96951

Purpose

Retroviral plasmid containing mIFP for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Infrared Fluorescent Protein

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxKOk

Plasmid

#229949

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKOk

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pETSG

Plasmid

#159418

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Bacterial

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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YeNL_pRSETb

Plasmid

#89520

Purpose

Yellow color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Green Falcan1

Plasmid

#199705

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan1

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pL0M-S-mNeonGreen-EC18153

Plasmid

#137075

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBbS9a-RFP

Plasmid

#126172

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-luxKate

Plasmid

#229946

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKate

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)

Plasmid

#173454

Purpose

Contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-rDAD2 cPGFP(G164)

Plasmid

#165994

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rDAD2 cPGFP(G164)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJS119

Plasmid

#207592

Purpose

GFP-TonB deltaII expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TonB deltaII

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJS104

Plasmid

#207589

Purpose

GFP-TolA deltaII expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TolA deltaII

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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