We narrowed to 3,906 results for: SPL

1. 35S-GWY-GAL4BD/pAM

   Plasmid

   #201231

   Purpose

   Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

   Depositor

   Michael Ayliffe

   Article

   Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

   Insert

   35S-GWY-GAL4BD
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 2Flag-pDEST-N

   Plasmid

   #118371

   Purpose

   Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   2Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EF1a-dCasRx-RBM25

   Plasmid

   #221001

   Purpose

   Transient expression of dCasRx-RBM25. EF1a promoter.

   Depositor

   Mikko Taipale, Benjamin Blencowe

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   dCasRx-RBM25
   

   Use

   CRISPR

   Tags

   NLS and NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 2Flag-pDEST-C

   Plasmid

   #118372

   Purpose

   Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   2Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CaVα2δ1

   Plasmid

   #26575

   Depositor

   Diane Lipscombe

   Article

   Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

   Insert

   Cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP_sfCherry2(11)_HP1

   Plasmid

   #82607

   Purpose

   Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_HP1b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Gly12 to Ala in sfCherry11+

   Promoter

   Available since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

7. GFP SMG1 WT

   Plasmid

   #199571

   Purpose

   Expresses WT SMG1 fused to GFP

   Depositor

   Michael Kastan

   Article

   McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

   Insert

   SMG1 WT (SMG1 Human)
   

   Use

   Tags

   GFP fusion protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET15b-hnRNP-H

   Plasmid

   #23020

   Depositor

   Douglas Black

   Article

   Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

   Insert

   hnRNP-H (HNRNPH1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-GFP1-10-pDEST-C

   Plasmid

   #118369

   Purpose

   Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   HA-GFP1-10

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-pEF1a-tTAc

    Plasmid

    #172123

    Purpose

    Constitutive expression of InteinC-tTAC

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    InteinC-tTAc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid

    #140220

    Purpose

    Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    dLbCpf1
    

    Use

    CRISPR

    Tags

    miniVPR-mCherry

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HA-GFP1-10-pDEST-N

    Plasmid

    #118368

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-GFP1-10

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTara:500

    Plasmid

    #60717

    Purpose

    Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pACUH-GFP11x7-mCherry-α-tubulin

    Plasmid

    #70218

    Purpose

    Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP11x7-mCherry-α-tubulin
    

    Use

    Tags

    GFP11x7 and mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. His-PIMT

    Plasmid

    #34852

    Depositor

    Bruce Downie

    Article

    Dinkins et al Plant J. 2008 Jul;55(1):1-13.

    Insert

    PIMT (PCMT1 Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pF3BGX-7xGFP11

    Plasmid

    #138397

    Purpose

    RMCE vector for generating C-terminal fusion of seven tandem GFP11 tags (split GFP)

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    7xGFP11
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG SMG1 WT

    Plasmid

    #199573

    Purpose

    Expresses FLAG-tagged WT SMG1

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 (SMG1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YFP-Rac1

    Plasmid

    #11391

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SpyTag-sfGFP

    Plasmid

    #184226

    Purpose

    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpyTag-sfGFP
    

    Use

    Tags

    His6, SpyTag, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FRB_SCF2_LD6_CTEV

    Plasmid

    #58879

    Purpose

    MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge