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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,081 results for: Vars

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Showing: 1861 - 1880 of 12081 results

  1. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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  3. pcDNA3.3_SARS2_EG.5.1

    Plasmid
    #207641
    Purpose
    pseudovirus plasmid that expresses spike of EG.5.1
    Depositor
    David Nemazee
    Insert
    Spike (EG.5.1) (S SARS-CoV-2)
    Expression
    Mammalian
    Mutation
    Q52H, F456L on the basis of XBB.1.5
    Promoter
    CMV
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    207641-plasmid-map-sequence-id-437731

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  5. pICSL60025

    Plasmid
    #245673
    Purpose
    Level 0 Golden Gate part Rb7MAR (D144-437 variant) terminator, GCTT - CGCT
    Depositor
    Mark Youles
    Insert
    Rb7MAR (D144-437 variant) terminator
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245673-plasmid-map-sequence-id-495495

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  6. pGMC00024 (aka pCW-Cas9n-Blast)

    Plasmid
    #195315
    Purpose
    Dox inducible lentiviral Cas9 nickase
    Depositor
    Raj Chari
    Insert
    Cas9-nickase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A nickase
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    195315-plasmid-map-sequence-id-390525

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  7. pCas9CR5

    Plasmid
    #120227
    Purpose
    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
    Depositor
    Christopher Reisch
    Insert
    SpCas9
    Use
    Synthetic Biology
    Mutation
    Stop codon inserted before ssrA degradation tag
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120227-plasmid-map-sequence-id-233288

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  8. pJK505

    Plasmid
    #71594
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, additiona…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71594-plasmid-map-sequence-id-131669

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  9. pJK506

    Plasmid
    #71596
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-165 to phenylalanine, addition…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71596-plasmid-map-sequence-id-131670

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  10. pGEX-6p1-GST-Ubiquitin

    Plasmid
    #223236
    Purpose
    Bacterial expression construct for GST-Tagged Ubiquitin production and purification
    Depositor
    Weixing Zhao
    Insert
    Ubiquitin
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223236-plasmid-map-sequence-id-443802

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  11. pBabe-CD44v6

    Plasmid
    #107970
    Purpose
    expression of CD44 variant
    Depositor
    Georg Weber
    Insert
    mCD44v6 (Cd44 Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    splice variant; see Depositor Comments below
    Available Since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    107970-plasmid-map-sequence-id-218212

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  14. Mek2

    Plasmid
    #40776
    Depositor
    Dustin Maly
    Insert
    MKK2 (MAP2K2 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Starting Met removed
    Promoter
    CMV
    Available Since
    Oct. 15, 2012
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3.3_SARS2_BA.2.86

    Plasmid
    #208441
    Purpose
    expressing SARS-CoV-2 BA.2.86 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (BA.2.86) (S SARS-CoV-2)
    Expression
    Mammalian
    Mutation
    see GISAID sequence
    Promoter
    CMV
    Available Since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    208441-plasmid-map-sequence-id-410934

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  16. pBabe-CD44v4

    Plasmid
    #107969
    Purpose
    expression of CD44 variant
    Depositor
    Georg Weber
    Insert
    mCD44v4 (Cd44 Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    splice variant; see Depositor Comments below
    Available Since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    107969-plasmid-map-sequence-id-222222

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  17. pDON-AI-Npm2(N533)

    Plasmid
    #51475
    Purpose
    Express mouse phosphorylation-mimicking Npm2 (p-Npm, N533)
    Depositor
    Shunsuke Ishii
    Insert
    Nucleoplasmin 2 (Npm2 Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    Eleven codons (5, 6, 7, 8, 10, 82, 95, 104, 184, …
    Available Since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51475-plasmid-map-sequence-id-78547

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  18. pCas9CR4-VQR

    Plasmid
    #120224
    Purpose
    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015
    Depositor
    Christopher Reisch
    Insert
    SpCas9-VQR
    Use
    Synthetic Biology
    Tags
    ssrA
    Mutation
    Mutations D1135V/R1335V/T1337R
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120224-plasmid-map-sequence-id-233296

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  20. pBabe-CD44v7

    Plasmid
    #107971
    Purpose
    expression of CD44 variant
    Depositor
    Georg Weber
    Insert
    mCD44v7 (Cd44 Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    splice variant; see Depositor Comments below
    Available Since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    107971-plasmid-map-sequence-id-212867

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Showing: 1861 - 1880 of 12081 results