We narrowed to 2,799 results for: fas

1. pC3.1_CAG_oROS-Gr_LF(C199S)

   Plasmid

   #216114

   Purpose

   Expresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-Gr_LF(C199S)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

   Plasmid

   #226514

   Purpose

   Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. HDAC8 Flag

   Plasmid

   #13825

   Depositor

   Eric Verdin

   Article

   Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

   Insert

   HDAC8 (HDAC8 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

   Plasmid

   #113884

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Promoter

   pHR-CMV-TetO2

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-dLight1.4

   Plasmid

   #111057

   Purpose

   Expresses dLight1.4 in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   dLight1.4
   

   Tags

   Flag tag

   Expression

   Mammalian

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

   Plasmid

   #113885

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

   Plasmid

   #226516

   Purpose

   Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   TOMM20-SNAP-tag2-T2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

   Plasmid

   #226515

   Purpose

   Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   Cox8a(4x)-SNAP-tag2-T2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-dLight1.3b

   Plasmid

   #111056

   Purpose

   Expresses dLight1.3b in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   dLight1.3b
   

   Tags

   Flag tag

   Expression

   Mammalian

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

    Plasmid

    #113888

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. cycLuc_TEVS

    Plasmid

    #119207

    Purpose

    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with TEVs cleavage site
    

    Use

    Luciferase

    Tags

    6xHis and AU1 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Myc-RILPL1

    Plasmid

    #201470

    Purpose

    For overexpression in cultured cells to visualize RILPL1 protein interactions.

    Depositor

    Suzanne Pfeffer

    Article

    Pal et al Sci Adv. 2023 Dec 15;9(50):eadj1205. doi

    Insert

    RILPL1 (RILPL1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

    Plasmid

    #189752

    Purpose

    Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mTurquoise2_spatial-linker-P2A_mScarlet3
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. FAB(mito-LD)

    Plasmid

    #214425

    Purpose

    Detection of mito-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspAN-3xHA-V5-mito
    

    Tags

    CFAST-Halo and RspAN-3XHA-V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a His6HuR

    Plasmid

    #135943

    Purpose

    Expresses HuR in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR-HOT-Clover-BlastR

    Plasmid

    #138569

    Purpose

    Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    Clover-BlastR
    

    Use

    CRISPR

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pExpreS2-1-C3-10H-SIII

    Plasmid

    #175444

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Tags

    3C-10His-TwinStrep and BiP Secretion Peptide

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

    Plasmid

    #189755

    Purpose

    Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mTurquoise2_spatial-linker-P2A_mScarlet-I3
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-dLight1.1

    Plasmid

    #111053

    Purpose

    Expresses dLight1.1 in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.1
    

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-CMV-TetO2_HA-BirA-ER

    Plasmid

    #113897

    Purpose

    Mammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    E. coli biotin ligase
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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